Ethyl acetate extract from Angelica Dahuricae Radix inhibits lipopolysaccharide-induced production of nitric oxide, prostaglandin E 2 and tumor necrosis factor-α via mitogen-activated protein kinases and nuclear factor-κB in macrophages

• Published in: Pharmacological research Vol. 55; no. 4; pp. 263 - 270
• Main Authors: Kang, Ok-Hwa, Lee, Go-Hoon, Choi, Hyuk Joon, Park, Pil Sang, Chae, Hee-Sung, Jeong, Seung-Il, Kim, Youn-Chul, Sohn, Dong Hwan, Park, Hyun, Lee, John Hwa, Kwon, Dong-Yeul
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.04.2007
• Subjects: Angelica Dahuricae Radix; Cyclooxygenase-2; Inducible nitric oxide synthase; Mitogen-activated protein kinase (MAPK); Nuclear factor-κB; Cyclooxygenase-2; Nuclear factor-κB; Angelica Dahuricae Radix; Inducible nitric oxide synthase; Mitogen-activated protein kinase (MAPK)
• ISSN: 1043-6618; 1096-1186
• DOI: 10.1016/j.phrs.2006.12.001

Angelica dahurica (Umbelliferae) has been used to treat headache of common cold, supraorbital neuralgia, painful swelling on the body, nasal stuffiness, leukorrhea and arthralgia due to wind-dampness in Korean traditional medicine. It is also claimed to be effective in the treatment of acne, erythema, headache, toothache, sinusitis, colds and flu. The present study focused whether the ethyl acetate extract from Angelica Dahuricae Radix (EAAD) inhibits production of nitric oxide (NO), prostaglandin E 2 (PGE 2) and tumor necrosis factor (TNF)-α, as well as expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPKs) in lipopolysaccharide (LPS)-stimulated macrophages. EAAD inhibited LPS-induced NO, PGE 2 and TNF-α production as well as expression of iNOS and COX-2 in RAW 264.7 cells. EAAD inhibited LPS-induced TNF-α production in THP-1 cells. Furthermore, EAAD suppressed LPS-induced phosphorylation of p38 MAPK and extracellular-signal regulated kinases 1/2 (ERK1/2), I-κBα degradation, and NF-κB activation in RAW 264.7 cells. These results suggest that EAAD has the inhibitory effects on LPS-induced TNF-α, NO and PGE 2 production, and expression of iNOS and COX-2 in macrophage through blockade in the phosphorylation of MAPKs, following I-κBα degradation and NF-κB activation.