Plasma β-amyloid1–42 reference values in cognitively normal subjects

• Published in: Journal of the neurological sciences Vol. 391; pp. 120 - 126
• Main Authors: Zecca, Chiara, Tortelli, Rosanna, Panza, Francesco, Arcuti, Simona, Piccininni, Marco, Capozzo, Rosa, Barulli, Maria Rosaria, Barone, Roberta, Cardinali, Roberta, Abbrescia, Daniela, Seripa, Davide, Brescia, Vincenzo, Logroscino, Giancarlo
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.08.2018
• Subjects: Alzheimer's disease; Aβ peptide; Biomarker; Plasma; Reference values; β-amyloid; APP; Plasma; Biomarker; Aβ1–40; ALT; DSM-IV-TR; DP; Reference values; CDR; Aβ; SD; ANOVA; CSF; Hb; IC; Aβ1–42; SP; RIs; Alzheimer's disease; IFCC; BMI; β-amyloid; CLSI; AST; AD; Aβ peptide; ESR; HOMA Index; TSH; NINCDS-ADRDA; EDTA; MCI; CV; UPDRS-III; GGT; SPs; PET; ELISA
• ISSN: 0022-510X; 1878-5883; 1878-5883
• DOI: 10.1016/j.jns.2018.06.006

In clinical practice, the use of plasma β-Amyloid1–42 (Aβ1–42) as biomarker for Alzheimer's disease is largely limited by the absence of reference values. The aim of this study was to evaluate Aβ1–42 plasma concentrations in cognitively normal subjects and to propose reference values. Plasma samples were obtained from 245 subjects, with a wide age-range (19–89 years), enrolled at the Unit of Laboratory Medicine of the “Azienda Ospedaliera Cardinale G. Panico” (younger subjects) and from a population-based study on aging (GreatAGE study) (older subjects). Three different age-groups were established: young (≤ 34), adult (35 ≤ age ≤ 64) and old (>64). The Innogenetics Elisa kit for plasma Aβ1–42 was used for the analysis. The mean (SD) concentration of plasma Aβ1–42 was 17.65 (5.71) pg/mL. A positive trend was found in Aβ1–42 levels across the three age groups (p < .0001): young subjects showed values of Aβ1–42 significantly lower than the adult group (p < .0001) and than the old one (p < .0001 overall); no significant differences were found between the adult and the old groups (p = 1.0000). For the entire cohort the lower limit of 90% Reference Interval, double-sided, was 8.12 pg/mL (95% CI 6.77–9.45) and the upper limit was 29.00 pg/mL (95% CI 27.01–31.00). The present study proposes reference values for plasma Aβ1–42. Nevertheless, further studies are needed to confirm these results and corroborate the use of these reference values in clinical practice. •Aβ1–42 plasma levels evaluated in cognitively normal subjects.•Plasma samples obtained from 245 subjects, 19–89 years of age.•Three different age groups established: age ≤ 34 years, 35 ≤ age ≤ 64 years, age > 64.•Significant differences among the three age subgroups.