Isolation and characterization of a Bacillus subtilis strain with aflatoxin B1 biodegradation capability
Aflatoxins are type of mycotoxins mainly produced by Aspergillus flavus and a common contaminant of food and grain, posing a serious economic and health problem worldwide. In order to find efficient bacteria to remove or detoxify these mycotoxins, a bacterial strain capable of degrading aflatoxin B1...
Saved in:
Published in | Food control Vol. 75; pp. 92 - 98 |
---|---|
Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Ltd
01.05.2017
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Aflatoxins are type of mycotoxins mainly produced by Aspergillus flavus and a common contaminant of food and grain, posing a serious economic and health problem worldwide. In order to find efficient bacteria to remove or detoxify these mycotoxins, a bacterial strain capable of degrading aflatoxin B1 (AFB1) was isolated from soil samples using a culture medium containing coumarin as the sole carbon source. Based on 16S rRNA gene sequence analysis, this isolate was identified as Bacillus subtilis JSW-1; its further characterization showed that it could inhibit the growth of A. flavus with an inhibition ratio of 58.3% and could degrade AFB1 by 67.2% after incubation at 30 °C for 72 h. The aflatoxin B1-degrading activity of isolate JSW-1 was predominantly attributed to the cell-free supernatant and this activity was found to be heat stable but sensitive to proteinase K treatment, indicating that the extracellular proteins or enzymes are responsible for the AFB1 degradation. In addition, no degradation products of AFB1 could be detected by liquid chromatography-mass spectrometry (LC-MS) analysis, indicating that the parent AFB1 might be biotransformed to compounds with chemical properties different from that of AFB1.
•B. subtilis JSW-1 capable of AFB1 degradation was isolated and identified.•Isolate of JSW-1 exhibits growth inhibition on A. flavus.•Extracellular proteins of B. subtilis JSW-1 are responsible for AFB1 degradation.•No degradation product of AFB1 can be detected by HPLC or LC-MS. |
---|---|
ISSN: | 0956-7135 1873-7129 |
DOI: | 10.1016/j.foodcont.2016.12.036 |