Revisionist look at solvophobic driving forces in reversed-phase liquid chromatography: II. Partitioning vs. adsorption mechanism in monomeric alkyl bonded phase supports

The partitioning and adsorption mechanisms of retention in reversed-phase liquid chromatography (RPLC) were examined based on the free energy of transfer of a methylene group from organic aqueous mixtures to bulk hexadecane. Retention on monomeric bonded phases with octyl chains or longer are domina...

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Bibliographic Details
Published inJournal of Chromatography A Vol. 775; no. 1-2; pp. 1 - 12
Main Authors Tan, Lay Choo, Carr, Peter W
Format Journal Article
LanguageEnglish
Published Elsevier B.V 18.07.1997
Subjects
Solvophobic theory
Partitioning
Adsorption
Retention mechanisms
Stationary phases, LC
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Summary:The partitioning and adsorption mechanisms of retention in reversed-phase liquid chromatography (RPLC) were examined based on the free energy of transfer of a methylene group from organic aqueous mixtures to bulk hexadecane. Retention on monomeric bonded phases with octyl chains or longer are dominated by a partitioning-like mechanism. An adsorption-like mechanism contributes to retention in monomeric bonded phases with short bonded chains or with low surface coverage density. The retention mechanism becomes more adsorption-like as the length of the bonded chains decrease.
ISSN:0021-9673
DOI:10.1016/S0021-9673(97)00228-8