Action du Facteur de croissance nerveuse (Nerve Growth Factor) sur la différenciation de cellules embryonnaires d'amphibien

• Published in: Experimental cell research Vol. 68; no. 1; pp. 25 - 32
• Main Authors: Mathieu, C., Duprat, A.-M., Zalta, J.-P., Beetschen, J.-C.
• Format: Journal Article
• Language: French
• Published: Elsevier Inc 1971
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1016/0014-4827(71)90582-9

The in vitro effects of NGF extract on determinate and indeterminate cultured cells of Pleurodeles waltlii ((Amphibian, Urodela) are studied. Cytological changes at nuclear and cytoplasmic levels are observed. The formation of neurons induced by NGF in undeterminated cell cultures (young gastrula's presumptive ventral epiblast), plus the presence of a particular cell type in determinated cell cultures (young neurula's neuroblast and chrodomesoblast) are described. We witnessed for this factor a neuron growth stimulating effect. The cytological alterations and the effects on morphological differentiation are irreversible. A few hypotheses for NGF mechanism are proposed.