수용성 누에 실샘 가수분해물의 미백 및 항노화 효과

• Published in: KSBB Journal Vol. 28; no. 3; pp. 196 - 201
• Main Authors: 정지혜, 황정욱, 차현명, 김호진, 김동일, 최용수
• Format: Journal Article
• Language: Korean
• Published: 한국생물공학회 2013
• Subjects: 생물공학; Whitening; Anti-aging; ROS; Silkworm gland hydrolysate
• ISSN: 1225-7117; 2288-8268

Silkworm-derived silk materials which contain 45 to 55% protein (18 amino acids, including 8 essential amino acids) have free radical-scavenging activities. In the present study, we investigated the whitening and anti-aging effects of silkworm gland under various conditions with B16F1 melanoma cells and human dermal fibroblasts. To evaluate the toxicity of soluble gland hydrolysate (SGH), mouse-derived B16F1 melanoma cells were treated with 0.2~5 mg/mL, cytotoxicity was not observed at all concentrations. The tyrosinase and elastase activities were significantly decreased as dosage levels of SGH increased. In addition, cell death was decreased by treated with SGH and antioxidative activity was the most effective in the SGH treatment. These findings suggest that SGH may be used as a potential functional biomaterial in having the skin whitening effect and anti-aging activity by inhibition of tyrosinase and elastase activities.