MMP-9 Sheds the β2 Integrin Subunit (CD18) from MacrophagesS

Activated macrophages are essential effectors of immunity and a rich source of matrix metalloproteinase-9 (MMP-9; gelatinase B). To search for cellular substrates of the enzyme, we subjected wild-type macrophages and macrophages expressing an autoactivating form of pro-MMP-9 (M9A macrophages) to pro...

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Published inMolecular & cellular proteomics Vol. 8; no. 5; pp. 1044 - 1060
Main Authors Vaisar, Tomáš, Kassim, Sean Y., Gomez, Ivan G., Green, Pattie S., Hargarten, Sara, Gough, Peter J., Parks, William C., Wilson, Carole L., Raines, Elaine W., Heinecke, Jay W.
Format Journal Article
LanguageEnglish
Published Elsevier Inc 01.05.2009
American Society for Biochemistry and Molecular Biology
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Abstract Activated macrophages are essential effectors of immunity and a rich source of matrix metalloproteinase-9 (MMP-9; gelatinase B). To search for cellular substrates of the enzyme, we subjected wild-type macrophages and macrophages expressing an autoactivating form of pro-MMP-9 (M9A macrophages) to proteomics analysis. Two-dimensional liquid chromatography together with tandem mass spectrometry identified 467 proteins in medium conditioned by M9A and/or wild-type macrophages. Subtractive proteomics identified 18 candidate MMP-9 substrates. Biochemical studies confirmed that two transmembrane proteins, β2 integrin subunit (CD18) and amyloid protein precursor (APP), were enriched in the medium of M9A macrophages. To identify potential cleavage sites, we synthesized an overlapping library of peptides that spanned 60 residues of the ectodomain and transmembrane domain of β2 integrin. Active MMP-9 cleaved a single peptide, ECVKGPNVAAIVGGT, at residues corresponding to Ala705 and Ile706 of the β2 integrin. Peptides corresponding to this cleavage site were detected by tandem mass spectrometric analysis only in medium from M9A macrophages, strongly supporting the proposal that β2 integrin is shed by autoactivating MMP-9. Our observations indicate that subtractive proteomics in concert with peptide substrate mapping is a powerful approach for identifying proteolytic substrates and suggest that MMP-9 plays previously unsuspected roles in the regulation and shedding of β2 integrin.
AbstractList Activated macrophages are essential effectors of immunity and a rich source of matrix metalloproteinase-9 (MMP-9; gelatinase B). To search for cellular substrates of the enzyme, we subjected wild-type macrophages and macrophages expressing an autoactivating form of pro-MMP-9 (M9A macrophages) to proteomics analysis. Two-dimensional liquid chromatography together with tandem mass spectrometry identified 467 proteins in medium conditioned by M9A and/or wild-type macrophages. Subtractive proteomics identified 18 candidate MMP-9 substrates. Biochemical studies confirmed that two transmembrane proteins, β 2 integrin subunit (CD18) and amyloid protein precursor (APP), were enriched in the medium of M9A macrophages. To identify potential cleavage sites, we synthesized an overlapping library of peptides that spanned 60 residues of the ectodomain and transmembrane domain of β 2 integrin. Active MMP-9 cleaved a single peptide, ECVKGPNVAAIVGGT, at residues corresponding to Ala 705 and Ile 706 of the β 2 integrin. Peptides corresponding to this cleavage site were detected by tandem mass spectrometric analysis only in medium from M9A macrophages, strongly supporting the proposal that β 2 integrin is shed by autoactivating MMP-9. Our observations indicate that subtractive proteomics in concert with peptide substrate mapping is a powerful approach for identifying proteolytic substrates and suggest that MMP-9 plays previously unsuspected roles in the regulation and shedding of β 2 integrin.
Activated macrophages are essential effectors of immunity and a rich source of matrix metalloproteinase-9 (MMP-9; gelatinase B). To search for cellular substrates of the enzyme, we subjected wild-type macrophages and macrophages expressing an autoactivating form of pro-MMP-9 (M9A macrophages) to proteomics analysis. Two-dimensional liquid chromatography together with tandem mass spectrometry identified 467 proteins in medium conditioned by M9A and/or wild-type macrophages. Subtractive proteomics identified 18 candidate MMP-9 substrates. Biochemical studies confirmed that two transmembrane proteins, β2 integrin subunit (CD18) and amyloid protein precursor (APP), were enriched in the medium of M9A macrophages. To identify potential cleavage sites, we synthesized an overlapping library of peptides that spanned 60 residues of the ectodomain and transmembrane domain of β2 integrin. Active MMP-9 cleaved a single peptide, ECVKGPNVAAIVGGT, at residues corresponding to Ala705 and Ile706 of the β2 integrin. Peptides corresponding to this cleavage site were detected by tandem mass spectrometric analysis only in medium from M9A macrophages, strongly supporting the proposal that β2 integrin is shed by autoactivating MMP-9. Our observations indicate that subtractive proteomics in concert with peptide substrate mapping is a powerful approach for identifying proteolytic substrates and suggest that MMP-9 plays previously unsuspected roles in the regulation and shedding of β2 integrin.
Author Raines, Elaine W.
Kassim, Sean Y.
Gough, Peter J.
Heinecke, Jay W.
Gomez, Ivan G.
Green, Pattie S.
Vaisar, Tomáš
Hargarten, Sara
Wilson, Carole L.
Parks, William C.
AuthorAffiliation From the Departments of ‡ Medicine and ¶ Pathology, School of Medicine, University of Washington, Seattle, Washington 98195
AuthorAffiliation_xml – name: From the Departments of ‡ Medicine and ¶ Pathology, School of Medicine, University of Washington, Seattle, Washington 98195
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  givenname: Ivan G.
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Notes Present address: GlaxoSmithKline, Collegeville, PA 19426.
To whom correspondence should be addressed: Division of Metabolism, Endocrinology, and Nutrition, Box 358055, University of Washington Medicine SLU, 815 Mercer, Seattle, WA 98109. E-mail: heinecke@u.washington.edu
Supported by a pilot award from the University of Washington Diabetes Endocrinology Research Center.
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References Van den Steen, Proost, Wuyts, Van Damme, Opdenakker (bib18) 2000; 96
Ito, Mukaiyama, Itoh, Nagase, Thogersen, Enghild, Sasaguri, Mori (bib87) 1996; 271
Weber (bib75) 2003; 81
Stefanidakis, Ruohtula, Borregaard, Gahmberg, Koivunen (bib78) 2004; 172
Overall, Dean (bib13) 2006; 25
Ortega, Behonick, Colnot, Cooper, Werb (bib85) 2005; 16
Bech-Serra, Santiago-Josefat, Esselens, Saftig, Baselga, Arribas, Canals (bib28) 2006; 26
Shipley, Wesselschmidt, Kobayashi, Ley, Shapiro (bib34) 1996; 93
Parks, Wilson, Lopez-Boado (bib3) 2004; 4
Keller, Nesvizhskii, Kolker, Aebersold (bib36) 2002; 74
Stinchcombe, Griffiths (bib57) 1999; 147
Lee, Corry (bib76) 2004; 10
Feutren, Lacour, Bach (bib46) 1984; 75
Stinchcombe, Griffiths (bib58) 2001; 103
Overall, Blobel (bib17) 2007; 8
Hwang, Park, Kim, Lee (bib24) 2004; 1702
Guo, Eisenman, Mahimkar, Peschon, Paxton, Black, Johnson (bib16) 2002; 1
Hiemstra, van den Barselaar, Roest, Nibbering, van Furth (bib56) 1999; 66
Parks, Lopez-Boado, Wilson (bib15) 2001; 120
Wild, Huang, Schulze-Horsel, van der Merwe, Vestweber (bib70) 2001; 276
Garton, Gough, Raines (bib67) 2006; 79
Park, Ni, Gerkema, Liu, Belozerov, Sang (bib20) 2000; 275
Old, Meyer-Arendt, Aveline-Wolf, Pierce, Mendoza, Sevinsky, Resing, Ahn (bib39) 2005; 4
Woessner, Nagase (bib1) 2000
Tam, Morrison, Wu, Stack, Overall (bib25) 2004; 101
Libby, Aikawa (bib8) 1998; 56
Schirmer, Florens, Guan, Yates, II, Gerace (bib31) 2003; 301
Sheu, Hsu, Ho, Lien, Huang, Lin (bib86) 2001; 61
Ong, Mann (bib54) 2005; 1
Greenlee, Corry, Engler, Matsunami, Tessier, Cook, Werb, Kheradmand (bib26) 2006; 177
Allinson, Parkin, Turner, Hooper (bib81) 2003; 74
Dieguez-Acuna, Gerber, Kodama, Elias, Beausoleil, Faustman, Gygi (bib30) 2005; 4
Postina (bib61) 2008; 5
Stipp, Kolesnikova, Hemler (bib71) 2003; 163
Dennis, Sherman, Hosack, Yang, Gao, Lane, Lempicki (bib48) 2003; 4
Mayadas, Cullere (bib69) 2005; 26
Dean, Overall (bib29) 2007; 6
Chelius, Bondarenko (bib43) 2002; 1
Vaisar, Pennathur, Green, Gharib, Hoofnagle, Cheung, Byun, Vuletic, Kassim, Singh, Chea, Knopp, Brunzell, Geary, Chait, Zhao, Elkon, Marcovina, Ridker, Oram, Heinecke (bib35) 2007; 117
Galis, Khatri (bib6) 2002; 90
Kulstad, Green, Cook, Watson, Reger, Baker, Plymate, Asthana, Rhoads, Mehta, Craft (bib47) 2006; 66
Yan, Hu, Song, Yin, Bateman, Cirrito, Xiao, Hsu, Turk, Xu, Hsu, Holtzman, Lee (bib83) 2006; 281
Thompson, Parks (bib12) 1996; 800
Lu, Vogel, Wang, Yao, Marcotte (bib40) 2007; 25
Bryceson, March, Ljunggren, Long (bib73) 2006; 214
Heissig, Hattori, Dias, Friedrich, Ferris, Hackett, Crystal, Besmer, Lyden, Moore, Werb, Rafii (bib84) 2002; 109
Huovila, Turner, Pelto-Huikko, Karkkainen, Ortiz (bib68) 2005; 30
Sondag, Combs (bib59) 2004; 279
Birkedal-Hansen, Moore, Bodden, Windsor, Birkedal-Hansen, DeCarlo, Engler (bib7) 1993; 4
Wang, Zhou, Lin, Roy, Shaler, Hill, Norton, Kumar, Anderle, Becker (bib44) 2003; 75
Hardy, Selkoe (bib79) 2002; 297
Green, Vaisar, Pennathur, Kulstad, Moore, Marcovina, Brunzell, Knopp, Zhao, Heinecke (bib42) 2008; 118
Yin, Cirrito, Yan, Hu, Xiao, Pan, Bateman, Song, Hsu, Turk, Xu, Hsu, Mills, Holtzman, Lee (bib82) 2006; 26
Mott, Werb (bib2) 2004; 16
Fu, Gharib, Green, Aitken, Frazer, Park, Vaisar, Heinecke (bib41) 2008; 7
Thompson (bib5) 1996; 11
Knauper, Cowell, Smith, Lopez-Otin, O'shea, Morris, Zardi, Murphy (bib21) 1997; 272
Garton, Ferri, Raines (bib32) 2002; 32
Bolshev (bib50) 1962; 7
Shapiro (bib4) 2003; 44
Brule, Charnaux, Sutton, Ledoux, Chaigneau, Saffar, Gattegno (bib52) 2006; 16
Talamagas, Efthimiopoulos, Tsilibary, Figueiredo-Pereira, Tzinia (bib80) 2007; 28
Washburn, Ulaszek, Yates, II (bib53) 2003; 75
Fernandes, Smith (bib55) 2002; 296
Nesvizhskii, Keller, Kolker, Aebersold (bib37) 2003; 75
Resing, Meyer-Arendt, Mendoza, Aveline-Wolf, Jonscher, Pierce, Old, Cheung, Russell, Wattawa, Goehle, Knight, Ahn (bib45) 2004; 76
Xu, Suenaga, Edelmann, Fridman, Muschel, Kessler (bib27) 2008; 7
La Fleur, Underwood, Rappolee, Werb (bib10) 1996; 184
De Strooper, Annaert (bib62) 2000; 113
Liu, Sadygov, Yates, II (bib38) 2004; 76
Hamerman, Ogasawara, Lanier (bib74) 2004; 172
Bauer, Konig, Strauss, Jonas, Ganter, Weidemann, Monning, Masters, Volk, Berger, Beyreuther (bib64) 1991; 282
Maere, Heymans, Kuiper (bib49) 2005; 21
Gough, Gomez, Wille, Raines (bib33) 2006; 116
Stefanidakis, Koivunen (bib60) 2006; 108
Owen, Hu, Barrick, Shapiro (bib14) 2003; 29
Evans, McDowall, Taylor, Hogg, Haskard, Landis (bib77) 2006; 107
Ley, Laudanna, Cybulsky, Nourshargh (bib65) 2007; 7
Pepper (bib9) 2001; 21
Fisher, Fei, Laird, Stock, Allen, Sahagan, Strick (bib51) 2002; 41
Overall, McQuibban, Clark-Lewis (bib23) 2002; 383
Cerwenka, Bakker, McClanahan, Wagner, Wu, Phillips, Lanier (bib72) 2000; 12
Overall (bib22) 2002; 22
McQuibban, Gong, Wong, Wallace, Clark-Lewis, Overall (bib19) 2002; 100
Mazzone, Ricevuti (bib66) 1995; 80
Backstrom, Lim, Cullen, Tokes (bib63) 1996; 16
Davies (bib11) 1996; 94
References_xml – volume: 4
  start-page: P3
  year: 2003
  ident: bib48
  article-title: DAVID: Database for Annotation, Visualization, and Integrated Discovery
  publication-title: Genome Biol.
  contributor:
    fullname: Lempicki
– year: 2000
  ident: bib1
  publication-title: Matrix metalloproteinases and TIMPs
  contributor:
    fullname: Nagase
– volume: 103
  start-page: 10
  year: 2001
  end-page: 16
  ident: bib58
  article-title: Normal and abnormal secretion by haemopoietic cells
  publication-title: Immunology
  contributor:
    fullname: Griffiths
– volume: 79
  start-page: 1105
  year: 2006
  end-page: 1116
  ident: bib67
  article-title: Emerging roles for ectodomain shedding in the regulation of inflammatory responses
  publication-title: J. Leukoc. Biol.
  contributor:
    fullname: Raines
– volume: 66
  start-page: 1506
  year: 2006
  end-page: 1510
  ident: bib47
  article-title: Differential modulation of plasma β-amyloid by insulin in patients with Alzheimer disease
  publication-title: Neurology
  contributor:
    fullname: Craft
– volume: 4
  start-page: 197
  year: 1993
  end-page: 250
  ident: bib7
  article-title: Matrix metalloproteinases: a review
  publication-title: Crit. Rev. Oral Biol. Med.
  contributor:
    fullname: Engler
– volume: 16
  start-page: 7910
  year: 1996
  end-page: 7919
  ident: bib63
  article-title: Matrix metalloproteinase-9 (MMP-9) is synthesized in neurons of the human hippocampus and is capable of degrading the amyloid-β peptide (1–40)
  publication-title: J. Neurosci.
  contributor:
    fullname: Tokes
– volume: 109
  start-page: 625
  year: 2002
  end-page: 637
  ident: bib84
  article-title: Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand
  publication-title: Cell
  contributor:
    fullname: Rafii
– volume: 279
  start-page: 14456
  year: 2004
  end-page: 14463
  ident: bib59
  article-title: Amyloid precursor protein mediates proinflammatory activation of monocytic lineage cells
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Combs
– volume: 184
  start-page: 2311
  year: 1996
  end-page: 2326
  ident: bib10
  article-title: Basement membrane and repair of injury to peripheral nerve: defining a potential role for macrophages, matrix metalloproteinases, and tissue inhibitor of metalloproteinases-1
  publication-title: J. Exp. Med.
  contributor:
    fullname: Werb
– volume: 1
  start-page: 252
  year: 2005
  end-page: 262
  ident: bib54
  article-title: Mass spectrometry-based proteomics turns quantitative
  publication-title: Nat. Chem. Biol.
  contributor:
    fullname: Mann
– volume: 25
  start-page: 117
  year: 2007
  end-page: 124
  ident: bib40
  article-title: Absolute protein expression profiling estimates the relative contributions of transcriptional and translational regulation
  publication-title: Nat. Biotechnol.
  contributor:
    fullname: Marcotte
– volume: 26
  start-page: 388
  year: 2005
  end-page: 395
  ident: bib69
  article-title: Neutrophil β2 integrins: moderators of life or death decisions
  publication-title: Trends Immunol.
  contributor:
    fullname: Cullere
– volume: 93
  start-page: 3942
  year: 1996
  end-page: 3946
  ident: bib34
  article-title: Metalloelastase is required for macrophage-mediated proteolysis and matrix invasion in mice
  publication-title: Proc. Natl. Acad. Sci. U. S. A.
  contributor:
    fullname: Shapiro
– volume: 281
  start-page: 24566
  year: 2006
  end-page: 24574
  ident: bib83
  article-title: Matrix metalloproteinase-9 degrades amyloid-β fibrils in vitro and compact plaques in situ
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Lee
– volume: 96
  start-page: 2673
  year: 2000
  end-page: 2681
  ident: bib18
  article-title: Neutrophil gelatinase B potentiates interleukin-8 tenfold by aminoterminal processing, whereas it degrades CTAP-III, PF-4, and GRO-α and leaves RANTES and MCP-2 intact
  publication-title: Blood
  contributor:
    fullname: Opdenakker
– volume: 41
  start-page: 8289
  year: 2002
  end-page: 8297
  ident: bib51
  article-title: Engineering autoactivating forms of matrix metalloproteinase-9 and expression of the active enzyme in cultured cells and transgenic mouse brain
  publication-title: Biochemistry
  contributor:
    fullname: Strick
– volume: 147
  start-page: 1
  year: 1999
  end-page: 6
  ident: bib57
  article-title: Regulated secretion from hemopoietic cells
  publication-title: J. Cell Biol.
  contributor:
    fullname: Griffiths
– volume: 80
  start-page: 161
  year: 1995
  end-page: 175
  ident: bib66
  article-title: Leukocyte CD11/CD18 integrins: biological and clinical relevance
  publication-title: Haematologica
  contributor:
    fullname: Ricevuti
– volume: 272
  start-page: 7608
  year: 1997
  end-page: 7616
  ident: bib21
  article-title: The role of the C-terminal domain of human collagenase-3 (MMP-13) in the activation of procollagenase-3, substrate specificity, and tissue inhibitor of metalloproteinase interaction
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Murphy
– volume: 116
  start-page: 59
  year: 2006
  end-page: 69
  ident: bib33
  article-title: Macrophage expression of active MMP-9 induces acute plaque disruption in apoE-deficient mice
  publication-title: J. Clin. Investig.
  contributor:
    fullname: Raines
– volume: 296
  start-page: 167
  year: 2002
  end-page: 171
  ident: bib55
  article-title: A novel antimicrobial function for a ribosomal peptide from rainbow trout skin
  publication-title: Biochem. Biophys. Res. Commun.
  contributor:
    fullname: Smith
– volume: 1
  start-page: 317
  year: 2002
  end-page: 323
  ident: bib43
  article-title: Quantitative profiling of proteins in complex mixtures using liquid chromatography and mass spectrometry
  publication-title: J. Proteome Res.
  contributor:
    fullname: Bondarenko
– volume: 76
  start-page: 3556
  year: 2004
  end-page: 3568
  ident: bib45
  article-title: Improving reproducibility and sensitivity in identifying human proteins by shotgun proteomics
  publication-title: Anal. Chem.
  contributor:
    fullname: Ahn
– volume: 61
  start-page: 237
  year: 2001
  end-page: 242
  ident: bib86
  article-title: A novel role of metalloproteinase in cancer-mediated immunosuppression
  publication-title: Cancer Res.
  contributor:
    fullname: Lin
– volume: 301
  start-page: 1380
  year: 2003
  end-page: 1382
  ident: bib31
  article-title: Nuclear membrane proteins with potential disease links found by subtractive proteomics
  publication-title: Science
  contributor:
    fullname: Gerace
– volume: 90
  start-page: 251
  year: 2002
  end-page: 262
  ident: bib6
  article-title: Matrix metalloproteinases in vascular remodeling and atherogenesis: the good, the bad, and the ugly
  publication-title: Circ. Res.
  contributor:
    fullname: Khatri
– volume: 75
  start-page: 5054
  year: 2003
  end-page: 5061
  ident: bib53
  article-title: Reproducibility of quantitative proteomic analyses of complex biological mixtures by multidimensional protein identification technology
  publication-title: Anal. Chem.
  contributor:
    fullname: II
– volume: 25
  start-page: 69
  year: 2006
  end-page: 75
  ident: bib13
  article-title: Degradomics: systems biology of the protease web. Pleiotropic roles of MMPs in cancer
  publication-title: Cancer Metastasis Rev.
  contributor:
    fullname: Dean
– volume: 177
  start-page: 7312
  year: 2006
  end-page: 7321
  ident: bib26
  article-title: Proteomic identification of in vivo substrates for matrix metalloproteinases 2 and 9 reveals a mechanism for resolution of inflammation
  publication-title: J. Immunol.
  contributor:
    fullname: Kheradmand
– volume: 21
  start-page: 3448
  year: 2005
  end-page: 3449
  ident: bib49
  article-title: BiNGO: a Cytoscape plugin to assess overrepresentation of gene ontology categories in biological networks
  publication-title: Bioinformatics
  contributor:
    fullname: Kuiper
– volume: 101
  start-page: 6917
  year: 2004
  end-page: 6922
  ident: bib25
  article-title: Membrane protease proteomics: isotope-coded affinity tag MS identification of undescribed MT1-matrix metalloproteinase substrates
  publication-title: Proc. Natl. Acad. Sci. U. S. A.
  contributor:
    fullname: Overall
– volume: 276
  start-page: 31602
  year: 2001
  end-page: 31612
  ident: bib70
  article-title: Affinity, kinetics, and thermodynamics of E-selectin binding to E-selectin ligand-1
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Vestweber
– volume: 297
  start-page: 353
  year: 2002
  end-page: 356
  ident: bib79
  article-title: The amyloid hypothesis of Alzheimer's disease: progress and problems on the road to therapeutics
  publication-title: Science
  contributor:
    fullname: Selkoe
– volume: 74
  start-page: 342
  year: 2003
  end-page: 352
  ident: bib81
  article-title: ADAMs family members as amyloid precursor protein α-secretases
  publication-title: J. Neurosci. Res.
  contributor:
    fullname: Hooper
– volume: 56
  start-page: 9
  year: 1998
  end-page: 13
  ident: bib8
  article-title: New insights into plaque stabilisation by lipid lowering
  publication-title: Drugs
  contributor:
    fullname: Aikawa
– volume: 16
  start-page: 488
  year: 2006
  end-page: 501
  ident: bib52
  article-title: The shedding of syndecan-4 and syndecan-1 from HeLa cells and human primary macrophages is accelerated by SDF-1/CXCL12 and mediated by the matrix metalloproteinase-9
  publication-title: Glycobiology
  contributor:
    fullname: Gattegno
– volume: 172
  start-page: 2001
  year: 2004
  end-page: 2005
  ident: bib74
  article-title: Cutting edge: Toll-like receptor signaling in macrophages induces ligands for the NKG2D receptor
  publication-title: J. Immunol.
  contributor:
    fullname: Lanier
– volume: 76
  start-page: 4193
  year: 2004
  end-page: 4201
  ident: bib38
  article-title: A model for random sampling and estimation of relative protein abundance in shotgun proteomics
  publication-title: Anal. Chem.
  contributor:
    fullname: II
– volume: 275
  start-page: 20540
  year: 2000
  end-page: 20544
  ident: bib20
  article-title: Identification and characterization of human endometase (matrix metalloproteinase-26) from endometrial tumor
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Sang
– volume: 6
  start-page: 611
  year: 2007
  end-page: 623
  ident: bib29
  article-title: Proteomics discovery of metalloproteinase substrates in the cellular context by iTRAQ labeling reveals a diverse MMP-2 substrate degradome
  publication-title: Mol. Cell. Proteomics
  contributor:
    fullname: Overall
– volume: 214
  start-page: 73
  year: 2006
  end-page: 91
  ident: bib73
  article-title: Activation, coactivation, and costimulation of resting human natural killer cells
  publication-title: Immunol. Rev.
  contributor:
    fullname: Long
– volume: 4
  start-page: 617
  year: 2004
  end-page: 629
  ident: bib3
  article-title: Matrix metalloproteinases as modulators of inflammation and innate immunity
  publication-title: Nat. Rev. Immunol.
  contributor:
    fullname: Lopez-Boado
– volume: 29
  start-page: 283
  year: 2003
  end-page: 294
  ident: bib14
  article-title: Inducible expression of tissue inhibitor of metalloproteinases-resistant matrix metalloproteinase-9 on the cell surface of neutrophils
  publication-title: Am. J. Respir. Cell Mol. Biol.
  contributor:
    fullname: Shapiro
– volume: 4
  start-page: 1459
  year: 2005
  end-page: 1470
  ident: bib30
  article-title: Characterization of mouse spleen cells by subtractive proteomics
  publication-title: Mol. Cell. Proteomics
  contributor:
    fullname: Gygi
– volume: 120
  start-page: 36S
  year: 2001
  end-page: 41
  ident: bib15
  article-title: Matrilysin in epithelial repair and defense
  publication-title: Chest
  contributor:
    fullname: Wilson
– volume: 30
  start-page: 413
  year: 2005
  end-page: 422
  ident: bib68
  article-title: Shedding light on ADAM metalloproteinases
  publication-title: Trends Biochem. Sci.
  contributor:
    fullname: Ortiz
– volume: 107
  start-page: 3593
  year: 2006
  end-page: 3599
  ident: bib77
  article-title: Shedding of lymphocyte function-associated antigen-1 (LFA-1) in a human inflammatory response
  publication-title: Blood
  contributor:
    fullname: Landis
– volume: 7
  start-page: 678
  year: 2007
  end-page: 689
  ident: bib65
  article-title: Getting to the site of inflammation: the leukocyte adhesion cascade updated
  publication-title: Nat. Rev. Immunol.
  contributor:
    fullname: Nourshargh
– volume: 7
  start-page: 2215
  year: 2008
  end-page: 2228
  ident: bib27
  article-title: Novel MMP-9 substrates in cancer cells revealed by a label-free quantitative proteomics approach
  publication-title: Mol. Cell. Proteomics
  contributor:
    fullname: Kessler
– volume: 26
  start-page: 5086
  year: 2006
  end-page: 5095
  ident: bib28
  article-title: Proteomic identification of desmoglein 2 and activated leukocyte cell adhesion molecule as substrates of ADAM17 and ADAM10 by difference gel electrophoresis
  publication-title: Mol. Cell. Biol.
  contributor:
    fullname: Canals
– volume: 163
  start-page: 1167
  year: 2003
  end-page: 1177
  ident: bib71
  article-title: EWI-2 regulates α3β1 integrin-dependent cell functions on laminin-5
  publication-title: J. Cell Biol.
  contributor:
    fullname: Hemler
– volume: 10
  start-page: 258
  year: 2004
  end-page: 262
  ident: bib76
  article-title: Homing alone? CD18 in infectious and allergic disease
  publication-title: Trends Mol. Med.
  contributor:
    fullname: Corry
– volume: 271
  start-page: 14657
  year: 1996
  end-page: 14660
  ident: bib87
  article-title: Degradation of interleukin 1β by matrix metalloproteinases
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Mori
– volume: 8
  start-page: 245
  year: 2007
  end-page: 257
  ident: bib17
  article-title: In search of partners: linking extracellular proteases to substrates
  publication-title: Nat. Rev. Mol. Cell Biol.
  contributor:
    fullname: Blobel
– volume: 21
  start-page: 1104
  year: 2001
  end-page: 1117
  ident: bib9
  article-title: Role of the matrix metalloproteinase and plasminogen activator-plasmin systems in angiogenesis
  publication-title: Arterioscler. Thromb. Vasc. Biol.
  contributor:
    fullname: Pepper
– volume: 4
  start-page: 1487
  year: 2005
  end-page: 1502
  ident: bib39
  article-title: Comparison of label-free methods for quantifying human proteins by shotgun proteomics
  publication-title: Mol. Cell. Proteomics
  contributor:
    fullname: Ahn
– volume: 800
  start-page: 157
  year: 1996
  end-page: 174
  ident: bib12
  article-title: Role of matrix metalloproteinases in abdominal aortic aneurysms
  publication-title: Ann. N. Y. Acad. Sci.
  contributor:
    fullname: Parks
– volume: 1
  start-page: 30
  year: 2002
  end-page: 36
  ident: bib16
  article-title: A proteomic approach for the identification of cell-surface proteins shed by metalloproteases
  publication-title: Mol. Cell. Proteomics
  contributor:
    fullname: Johnson
– volume: 113
  start-page: 1857
  year: 2000
  end-page: 1870
  ident: bib62
  article-title: Proteolytic processing and cell biological functions of the amyloid precursor protein
  publication-title: J. Cell Sci.
  contributor:
    fullname: Annaert
– volume: 26
  start-page: 10939
  year: 2006
  end-page: 10948
  ident: bib82
  article-title: Matrix metalloproteinases expressed by astrocytes mediate extracellular amyloid-β peptide catabolism
  publication-title: J. Neurosci.
  contributor:
    fullname: Lee
– volume: 28
  start-page: 304
  year: 2007
  end-page: 315
  ident: bib80
  article-title: Aβ(1–40)-induced secretion of matrix metalloproteinase-9 results in sAPPα release by association with cell surface APP
  publication-title: Neurobiol. Dis.
  contributor:
    fullname: Tzinia
– volume: 12
  start-page: 721
  year: 2000
  end-page: 727
  ident: bib72
  article-title: Retinoic acid early inducible genes define a ligand family for the activating NKG2D receptor in mice
  publication-title: Immunity
  contributor:
    fullname: Lanier
– volume: 11
  start-page: 504
  year: 1996
  end-page: 518
  ident: bib5
  article-title: Basic science of abdominal aortic aneurysms: emerging therapeutic strategies for an unresolved clinical problem
  publication-title: Curr. Opin. Cardiol.
  contributor:
    fullname: Thompson
– volume: 44
  start-page: 30s
  year: 2003
  end-page: 32s
  ident: bib4
  article-title: Proteolysis in the lung
  publication-title: Eur. Respir. J. Suppl.
  contributor:
    fullname: Shapiro
– volume: 117
  start-page: 746
  year: 2007
  end-page: 756
  ident: bib35
  article-title: Shotgun proteomics implicates protease inhibition and complement activation in the antiinflammatory properties of HDL
  publication-title: J. Clin. Investig.
  contributor:
    fullname: Heinecke
– volume: 75
  start-page: 4818
  year: 2003
  end-page: 4826
  ident: bib44
  article-title: Quantification of proteins and metabolites by mass spectrometry without isotopic labeling or spiked standards
  publication-title: Anal. Chem.
  contributor:
    fullname: Becker
– volume: 282
  start-page: 335
  year: 1991
  end-page: 340
  ident: bib64
  article-title: In-vitro matured human macrophages express Alzheimer's β A4-amyloid precursor protein indicating synthesis in microglial cells
  publication-title: FEBS Lett.
  contributor:
    fullname: Beyreuther
– volume: 118
  start-page: 1259
  year: 2008
  end-page: 1267
  ident: bib42
  article-title: Combined statin and niacin therapy remodels the high-density lipoprotein proteome
  publication-title: Circulation
  contributor:
    fullname: Heinecke
– volume: 32
  start-page: 830
  year: 2002
  ident: bib32
  article-title: Efficient expression of exogenous genes in primary vascular cells using IRES-based retroviral vectors
  publication-title: BioTechniques
  contributor:
    fullname: Raines
– volume: 7
  start-page: 113
  year: 1962
  end-page: 114
  ident: bib50
  article-title: On the comparison of parameters of Poisson distributions
  publication-title: Theory Probab. Appl.
  contributor:
    fullname: Bolshev
– volume: 81
  start-page: 4
  year: 2003
  end-page: 19
  ident: bib75
  article-title: Novel mechanistic concepts for the control of leukocyte transmigration: specialization of integrins, chemokines, and junctional molecules
  publication-title: J. Mol. Med.
  contributor:
    fullname: Weber
– volume: 16
  start-page: 558
  year: 2004
  end-page: 564
  ident: bib2
  article-title: Regulation of matrix biology by matrix metalloproteinases
  publication-title: Curr. Opin. Cell Biol.
  contributor:
    fullname: Werb
– volume: 5
  start-page: 179
  year: 2008
  end-page: 186
  ident: bib61
  article-title: A closer look at α-secretase
  publication-title: Curr. Alzheimer Res.
  contributor:
    fullname: Postina
– volume: 1702
  start-page: 79
  year: 2004
  end-page: 87
  ident: bib24
  article-title: A proteomic approach to identify substrates of matrix metalloproteinase-14 in human plasma
  publication-title: Biochim. Biophys. Acta
  contributor:
    fullname: Lee
– volume: 75
  start-page: 85
  year: 1984
  end-page: 94
  ident: bib46
  article-title: Immune lysis of hepatocytes in culture: accurate detection by aspartate aminotransferase release measurement
  publication-title: J. Immunol. Methods
  contributor:
    fullname: Bach
– volume: 75
  start-page: 4646
  year: 2003
  end-page: 4658
  ident: bib37
  article-title: A statistical model for identifying proteins by tandem mass spectrometry
  publication-title: Anal. Chem.
  contributor:
    fullname: Aebersold
– volume: 22
  start-page: 51
  year: 2002
  end-page: 86
  ident: bib22
  article-title: Molecular determinants of metalloproteinase substrate specificity: matrix metalloproteinase substrate binding domains, modules, and exosites
  publication-title: Mol. Biotechnol.
  contributor:
    fullname: Overall
– volume: 7
  start-page: 845
  year: 2008
  end-page: 854
  ident: bib41
  article-title: Spectral index for assessment of differential protein expression in shotgun proteomics
  publication-title: J. Proteome Res.
  contributor:
    fullname: Heinecke
– volume: 66
  start-page: 423
  year: 1999
  end-page: 428
  ident: bib56
  article-title: Ubiquicidin, a novel murine microbicidal protein present in the cytosolic fraction of macrophages
  publication-title: J. Leukoc. Biol.
  contributor:
    fullname: van Furth
– volume: 94
  start-page: 2013
  year: 1996
  end-page: 2020
  ident: bib11
  article-title: Stability and instability: two faces of coronary atherosclerosis. The Paul Dudley White Lecture 1995
  publication-title: Circulation
  contributor:
    fullname: Davies
– volume: 172
  start-page: 7060
  year: 2004
  end-page: 7068
  ident: bib78
  article-title: Intracellular and cell surface localization of a complex between αMβ2 integrin and promatrix metalloproteinase-9 progelatinase in neutrophils
  publication-title: J. Immunol.
  contributor:
    fullname: Koivunen
– volume: 100
  start-page: 1160
  year: 2002
  end-page: 1167
  ident: bib19
  article-title: Matrix metalloproteinase processing of monocyte chemoattractant proteins generates CC chemokine receptor antagonists with anti-inflammatory properties in vivo
  publication-title: Blood
  contributor:
    fullname: Overall
– volume: 74
  start-page: 5383
  year: 2002
  end-page: 5392
  ident: bib36
  article-title: Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search
  publication-title: Anal. Chem.
  contributor:
    fullname: Aebersold
– volume: 383
  start-page: 1059
  year: 2002
  end-page: 1066
  ident: bib23
  article-title: Discovery of chemokine substrates for matrix metalloproteinases by exosite scanning: a new tool for degradomics
  publication-title: Biol. Chem.
  contributor:
    fullname: Clark-Lewis
– volume: 108
  start-page: 1441
  year: 2006
  end-page: 1450
  ident: bib60
  article-title: Cell-surface association between matrix metalloproteinases: role of the complexes in leukocyte migration and cancer progression
  publication-title: Blood
  contributor:
    fullname: Koivunen
– volume: 16
  start-page: 3028
  year: 2005
  end-page: 3039
  ident: bib85
  article-title: Galectin-3 is a downstream regulator of matrix metalloproteinase-9 function during endochondral bone formation
  publication-title: Mol. Biol. Cell
  contributor:
    fullname: Werb
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Snippet Activated macrophages are essential effectors of immunity and a rich source of matrix metalloproteinase-9 (MMP-9; gelatinase B). To search for cellular...
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StartPage 1044
Title MMP-9 Sheds the β2 Integrin Subunit (CD18) from MacrophagesS
URI https://dx.doi.org/10.1074/mcp.M800449-MCP200
https://pubmed.ncbi.nlm.nih.gov/PMC2689762
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