MMP-9 Sheds the β2 Integrin Subunit (CD18) from MacrophagesS

• Published in: Molecular & cellular proteomics Vol. 8; no. 5; pp. 1044 - 1060
• Main Authors: Vaisar, Tomáš, Kassim, Sean Y., Gomez, Ivan G., Green, Pattie S., Hargarten, Sara, Gough, Peter J., Parks, William C., Wilson, Carole L., Raines, Elaine W., Heinecke, Jay W.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.05.2009; American Society for Biochemistry and Molecular Biology
• ISSN: 1535-9476; 1535-9484
• DOI: 10.1074/mcp.M800449-MCP200

Activated macrophages are essential effectors of immunity and a rich source of matrix metalloproteinase-9 (MMP-9; gelatinase B). To search for cellular substrates of the enzyme, we subjected wild-type macrophages and macrophages expressing an autoactivating form of pro-MMP-9 (M9A macrophages) to proteomics analysis. Two-dimensional liquid chromatography together with tandem mass spectrometry identified 467 proteins in medium conditioned by M9A and/or wild-type macrophages. Subtractive proteomics identified 18 candidate MMP-9 substrates. Biochemical studies confirmed that two transmembrane proteins, β2 integrin subunit (CD18) and amyloid protein precursor (APP), were enriched in the medium of M9A macrophages. To identify potential cleavage sites, we synthesized an overlapping library of peptides that spanned 60 residues of the ectodomain and transmembrane domain of β2 integrin. Active MMP-9 cleaved a single peptide, ECVKGPNVAAIVGGT, at residues corresponding to Ala705 and Ile706 of the β2 integrin. Peptides corresponding to this cleavage site were detected by tandem mass spectrometric analysis only in medium from M9A macrophages, strongly supporting the proposal that β2 integrin is shed by autoactivating MMP-9. Our observations indicate that subtractive proteomics in concert with peptide substrate mapping is a powerful approach for identifying proteolytic substrates and suggest that MMP-9 plays previously unsuspected roles in the regulation and shedding of β2 integrin.