Comparisons of Developmental Potential and Gene Expression Level in Porcine Nuclear Transfer, Parthenogenetic and Fertilized Embryos

• Published in: Reproductive & developmental biology Vol. 30; no. 2
• Main Authors: Kim, J.G. (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea), Kumar, B.M. (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea), Cho, S.K. (Gyeongsang National University, Jinju, Republic of Korea), Ock, S.A. (Gyeongsang National University, Jinju, Republic of Korea), Jeon, B.G. (Gyeongsang National University, Jinju, Republic of Korea), Balasubramanian, S. (TamilNadu Veterinary and Animal Sciences University, Chennai, India), Rho, G.J. (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea), Choe, S.Y. (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea), E-mail: sychoe@gsnu.ac.kr
• Format: Journal Article
• Language: English
• Published: 30.06.2006
• Subjects: APOPTOSE; APOPTOSIS; Gene expression; Nuclear transfer; Porcine
• ISSN: 1738-2432

This study was conducted to detect the apoptosis incidence in blastocysts and to compare the abundance of Bax, Bcl2L1, VEGF and FGFR2 in in vitro fertilized (IVF), parthenogenetic (PAT) and nuclear transfer (NT) embryos. Oocytes matured for 40 hr were enucleated and reconstructed with confluenced fetal fibroblasts (FFs) derived from a ~45 day fetus. Reconstructed eggs were then fused with 2 DC pulses (2.0 kV/cm, 30 μsec) and cultured with 7.5 ㎍/ml cytochalasin B for 3 hr. Parthenotes (PAT) were produced with the same electric strength and culture for NT eggs.