DETECTION OF RENIBACTERIUM SALMONINARUM IN TISSUE OF BROOK TROUT (SALVELINUS FONTINALIS) BY NESTED RT–PCR

Pathogenic bacterium Renibacterium salmoninarum causes kidney disease with high mortality rate and considerable economic losses in salmonid farming. Thus, application of fast and sensitive method for R. salmoninarum diagnosis is of great importance. This paper describes detection of R. salmoninarum...

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Published inRibarstvo Vol. 65; no. 1; pp. 15 - 24
Main Authors Irena Vardić, Damir Kapetanović, Damir Vailć, Božidar Kurtović, Zlatica Teskeredžić, Emin Teskeredžić
Format Journal Article
LanguageEnglish
Published Sciendo 01.01.2007
Subjects
brook trout
msa gene sequence
nested rt–pcr
renibacterium salmoninarum
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Summary:Pathogenic bacterium Renibacterium salmoninarum causes kidney disease with high mortality rate and considerable economic losses in salmonid farming. Thus, application of fast and sensitive method for R. salmoninarum diagnosis is of great importance. This paper describes detection of R. salmoninarum in brook trout tissue with gross clinical signs of disease by nested RT–PCR. Determination of partial sequence of bacterial msa gene was done prior to comparison with similar sequences from different R. salmoninarum isolates. Nested RT–PCR proved to be a rapid and valuable diagnostic tool for R. salmoninarum detection, and sequence analysis confirmed previously reported genetic uniformity of this bacteria
ISSN:1330-061X
1848-0586