ENCAPSULATION OF NAJA -NAJA OXIANA SNAKE VENOM INTO POLY (LACTIDE-CO-GLYCOLIDE) MICROSPHERES

One small-scale double emulsion technique for incorporation of Naja- Naja oxiana venom into Poly (lactide-co-glycolide) (PLGA) microspheres were developed and optimized. The effects of high speed homogenization on the double emulsion stability, microsphere size, entrapment efficiency and In vitro re...

Full description

Saved in:
Bibliographic Details
Published inArchives of pharmacy practice Vol. 3; no. 1; p. 10
Main Authors Zolfagharian, H, Dounighi, Mohammadpour N
Format Journal Article
LanguageEnglish
Published Selangor Monash University Malaysia, School of Pharmacy and Pharmaceutical Science 01.01.2012
Archives of Global Professionals
Subjects
Emulsion polymerization
Homogenization
Snakes
Venom
Iran
Online AccessGet full text

Cover

More Information
Summary:One small-scale double emulsion technique for incorporation of Naja- Naja oxiana venom into Poly (lactide-co-glycolide) (PLGA) microspheres were developed and optimized. The effects of high speed homogenization on the double emulsion stability, microsphere size, entrapment efficiency and In vitro release of venom were studied A stable double emulsion was verified by homogenization method. Slow removal of the organic phase allowed measurement of the size of the emulsion droplets and subsequent predication of the size which resulting microspheres. Microspheres in the size range of 1-10pm were prepared using homogenization technique, but this technique was sensitive to changes in the operating time, speed and volume of outer aqueous phase. Snake venom was released in vitro in a triphasic manner. After immunization of guineapig with a single IM injection, the PLGA-venom microspheres elicited an antibody response very high as that elicited with conventional method. These results indicate that the antigenicity of venom was retained after incorporation into PLGA microspheres using homogenization technique.
ISSN:2320-5210
2045-080X
2045-080X