Cyclin F Expression in Clear Cell Renal Cell Carcinoma and Its Effect on Biological Behavior of Renal Carcinoma Cell Lines

Objective To investigate the expression of Cyclin F in clear cell renal cell carcinoma （ccRCC）its clinicopathological characteristics,and its effect on the biological behavior of renal cancer cell line Methods RT-qPCR and Western blot were used to detect the mRNA and protein expression of Cyclin F i...

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Published inZhongliu fangzhi yanjiu Vol. 52; no. 6; pp. 474 - 480
Main Authors Su, Min, Wang, Yan, Hua, Jie, Wang, Tianyun, Xu, Shengnan, Kui, Xiang
Format Journal Article
LanguageChinese
English
Published Tianjin China Anti-Cancer Association 01.06.2025
Magazine House of Cancer Research on Prevention and Treatment
Subjects
Apoptosis
Assaying
biological behavior
Biological effects
Cancer
Cholecystokinin
clear cell renal cell carcinoma
Clear cell-type renal cell carcinoma
clinicopathology
Cyclin F
cyclins
F protein
Flow cytometry
Gene expression
Immunohistochemistry
Kidney cancer
mRNA
Proteins
Tissues
Tumor cell lines
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Summary:Objective To investigate the expression of Cyclin F in clear cell renal cell carcinoma （ccRCC）its clinicopathological characteristics,and its effect on the biological behavior of renal cancer cell line Methods RT-qPCR and Western blot were used to detect the mRNA and protein expression of Cyclin F in fresh ccRCC specimens.Immunohistochemistry assay was performed to detect the expression of Cyclin F protein in 80 paraffin samples.CCK-8 assay,scratch assay,and flow cytometry were conducted to determine the effects of Cyclin F overexpression on the proliferation,migration,and apoptosis of renal cancer cell lines Results The expression of Cyclin F in cancer tissues was higher than that in adjacent tissues at the mRNA level （P＜0.028 5）.The expression of Cyclin Fprotein in cancer tissues was lower than that in adjacent tissue（P＜0.001）.The analysis of clinicopathological parameters showed that the low expression of Cyclin F wa correlated with WHO/ISUP grade,Ki67 index,and age （P=0.041,0.047,0.008,）.In vitro experiment confirmed that overexpression of Cyclin F promoted the proliferation （P＜0.001） and migratory ability（24 h P=0.003,48 h P=0.005 8） of the RCC 786-O cell line,while inhibiting apoptosis （P=0.001 9）Conclusion The low expression of Cyclin F protein in ccRCC tissuesis associated with high ISUP grade high Ki67 index,oldage,and prognosis of patients.
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ISSN:1000-8578
DOI:10.3971/j.issn.1000-8578.2025.24.0553