Autophagy Related Protein 5 Regulates Colon Cancer Cell Senescence Through p53/p21 Pathway

• Published in: Zhongliu fangzhi yanjiu Vol. 46; no. 12; pp. 1068 - 1072
• Main Authors: Hu, Fuqing, Chen, Yaqi, Wu, Qi, Hu, Junbo, Wang, Guihua, Liu, Lu
• Format: Journal Article
• Language: Chinese
• Published: Tianjin China Anti-Cancer Association 01.12.2019; Magazine House of Cancer Research on Prevention and Treatment
• Subjects: atg5; Autophagy; Biotechnology; Cancer; cell proliferation; cell senescence; Cholecystokinin; Colon; Colon cancer; Colorectal cancer; Cyclin-dependent kinase inhibitor p21; Doxorubicin; Gene expression; GTP-binding protein; p53 Protein; Phagocytosis; Proteins; Senescence; siRNA; Transfection
• ISSN: 1000-8578; 1000-8578
• DOI: 10.3971/j.issn.1000-8578.2019.19.0416

Objective To investigate the effect of ATG5 on the senescence and proliferation of colon cancer HT29 cell line. Methods The senescence of colon cancer HT29 cell line was induced by doxorubicin. ATG5 expression was detected by Western blot. Low expression of ATG5 was detected by transient transfection of small interfering RNA, and the proportion of cell senescence and proliferation ability were detected by betagalactosidase staining and CCK assay, respectively. At the same time, we added Rapa to induce autophagy and detected the proportion of cell senescence and proliferation ability. The effect of ATG5 on cell senescence related pathway proteins P53 and P21 was detected by Western blot. Results The protein expression of ATG5 was down-regulated by doxorubicin-induced cell senescence. The mean number of senescent cells in the SiNC control group was 3±1, while that in the SiATG5 group was 12±2, and that in the low-expression ATG5 plus Rapa group was 12±1. Low expression of ATG5 could up-regulate the expression o