Study of Some Haematological Indices of Diploid and Triploid Rainbow Trout, Oncorhynchus mykiss

• Published in: Cell journal (Yakhteh) Vol. 11; no. 4; pp. 442 - 447
• Main Authors: Dorafshan, Salar, Kalbassi, Mohammad Reza, Karimi, Sahel Soltan, Rahimi, Khosrow
• Format: Journal Article
• Language: English
• Published: Tehran Royan Institute of Iran 01.01.2010; Royan Institute (ACECR), Tehran
• Subjects: Haematology; Oncorhynchus mykiss; Polyploidy; Rainbow trout
• ISSN: 2228-5806; 2228-5814

Objective: A study of the effects of triploidy on some haematological indices of rainbowtrout.Materials and Methods: Haematological characteristics such as red blood cell (RBC)dimensions, area and volume, RBC and white blood cell counts (WBC), haematocrit(Hct%), hemoglobin (Hb), mean erythrocytic hemoglobin (MEH), mean erythrocytic volume(MEV), mean erythrocytic hemoglobin concentration (MEHC) and RBC abnormalitieswere measured in 14 and 15 ten month old rainbow trout, respectively.Results: Triploidy significantly increased all morphometric indices containing dimensions,nuclear area and volume of RBCs in compare to diploid fish (p<0.05). The triploidtrout had lower numbers with larger RBC sizes. The decrease in RBCs was compensatedby an increase in MEV. Thus, triploidy did not affect Hct% (p>0.05). A significantreduction in Hb was observed in triploids (7.4 g/dL) when compared to diploid fish (9.2g/dL; p<0.05). The MEH values were 82.8 and 116.8 μg/cell in diploids and triploids, respectively(p<0.01). There were no significant differences in MEHC levels between diploidsand triploids (p>0.05). Triploids had lower numbers of WBCs and showed highererythrocytic abnormalities than diploids (p<0.01).Conclusion: Numerous haematological indices of rainbow trout were affected by theploidy levels, therefore it is expected that optimum culture conditions should vary betweendiploid and triploid fish.