Pepper (Capsicum annuum L.) cytoplasmic male sterilty
In the present study the molecular basis and mechanism of pepper cytoplasmic male sterility (CMS) and its restoration system (Rf) has been characterized in detail. Pollen fertility of five CMSs (No. 201 to 205), four restorer lines (No. 206, 207, 209 and 210) and their F-1, F-2 generations were inve...
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Published in | Journal of Central European agriculture Vol. 3; no. 2; pp. 149 - 158 |
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Main Authors | , , , |
Format | Journal Article |
Language | English Bulgarian |
Published |
University of Zagreb, Faculty of Agriculture
01.11.2002
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Subjects | |
Online Access | Get full text |
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Summary: | In the present study the molecular basis and mechanism of pepper cytoplasmic male sterility (CMS) and its restoration system (Rf) has been characterized in detail. Pollen fertility of five CMSs (No. 201 to 205), four restorer lines (No. 206, 207, 209 and 210) and their F-1, F-2 generations were investigated during different growing seasons to study the response of male sterility to various environmental conditions. Restorer gene specific primers were applied to reveal the molecular genetic differences between the CMS and restorer lines. "Bulk segregant" and individual analysis screened DNA markers linked to the fertility restorer (Rf) gene for cytoplasmic male sterility. By the application for conventional breeding and molecular genetic methods co-segregation of the restorer specific markers and pollen viability data were observed on hundred individuals of the F-2 generation in order to construct a physical linkage map. |
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Bibliography: | 2003010048 F30 |
ISSN: | 1332-9049 1332-9049 |