Genome-wide association analysis and transgenic characterization for amylose content regulating gene in tuber of Dioscorea zingiberensis

• Published in: BMC plant biology Vol. 24; no. 1; p. 524
• Main Authors: Sun, Shixian, Guan, Binbin, Xing, Yue, Li, Xiang, Liu, Lanlan, Li, Yanmei, Jia, Lu, Ye, Shili, Dossa, Komivi, Zheng, Li, Luan, Yunpeng
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 10.06.2024; BioMed Central; BMC
• Subjects: Adenosine diphosphate; ADP glucose pyrophosphorylase; Alleles; Amino acid sequence; Amylose; Amylose - metabolism; Analysis; Arabidopsis thaliana; Association analysis; Barley; Biochemistry, Molecular Biology; Biosynthesis; Cassava; Cereals; Chromosome 6; Chromosomes; Corn; Crops; Dextrose; Diabetes mellitus; Diabetics; Dietary fiber; Dioscorea - genetics; Dioscorea - metabolism; Enzymatic activity; Enzyme activity; Enzymes; Evaluation; Food products; Food quality; Food security; Food supply; Gene loci; Genes; Genes, Plant; Genetic aspects; Genetic diversity; Genetic engineering; Genetic research; Genetic variance; Genetically altered foods; Genetically modified organisms; Genetics; Genome wide association study; Genome-wide association studies; Genomes; Genomic analysis; Genomics; Genotypes; Glucose; Growth; Life Sciences; Microbiota (Symbiotic organisms); Microflora; Nucleotides; Physiological aspects; Plant Tubers - genetics; Plant Tubers - metabolism; Plants, Genetically Modified - genetics; Polymorphism, Single Nucleotide; Population structure; Populations and Evolution; Potatoes; Quantitative genetics; Rice; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Starch biosynthesis; Structural analysis; Tubers; Weight control; Wheat; Yams; China; Food quality; Genome wide association study; Food security; Yams; Starch biosynthesis
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-024-05122-4

Amylose, a prebiotic found in yams is known to be beneficial for the gut microflora and is particularly advantageous for diabetic patients' diet. However, the genetic machinery underlying amylose production remains elusive. A comprehensive characterization of the genetic basis of amylose content in yam tubers is a prerequisite for accelerating the genetic engineering of yams with respect to amylose content variation. To uncover the genetic variants underlying variation in amylose content, we evaluated amylose content in freshly harvested tubers from 150 accessions of Dioscorea zingibensis. With 30,000 high-quality single nucleotide polymorphisms (SNP), we performed a genome-wide association analysis (GWAS). The population structure analysis classified the D. zingiberensis accessions into three groups. A total of 115 significant loci were detected on four chromosomes. Of these, 112 significant SNPs (log10(p) = 5, q-value < 0.004) were clustered in a narrow window on the chromosome 6 (chr6). The peak SNP at the position 75,609,202 on chr6 could explain 63.15% of amylose variation in the population and fell into the first exon of the ADP-glucose pyrophosphorylase (AGPase) small subunit gene, causing a non-synonymous modification of the resulting protein sequence. Allele segregation analysis showed that accessions with the rare G allele had a higher amylose content than those harboring the common A allele. However, AGPase, a key enzyme precursor of amylose biosynthesis, was not expressed differentially between accessions with A and G alleles. Overexpression of the two variants of AGPase in Arabidopsis thaliana resulted in a significantly higher amylose content in lines transformed with the AGPase-G allele. Overall, this study showed that a major genetic variant in AGPase probably enhances the enzyme activity leading to high amylose content in D. zingiberensis tuber. The results provide valuable insights for the development of amylose-enriched genotypes.