Regional differences of Mycobacterium tuberculosis complex infection and multidrug resistance epidemic in Luoyang

• Published in: BMC infectious diseases Vol. 24; no. 1; pp. 578 - 11
• Main Authors: Wang, Zhenzhen, Guo, Tengfei, Xu, Liyang, Liu, Jinwei, Hou, Yi, Jin, Junrong, Zhang, Qing, Jiang, Tao, Zhao, Zhanqin, Xue, Yun
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 11.06.2024; BioMed Central; BMC
• Subjects: Acids; Adolescent; Adult; Aged; Aged, 80 and over; Antitubercular Agents - pharmacology; Antitubercular Agents - therapeutic use; Child; Child, Preschool; China - epidemiology; Comparative analysis; Control; COVID-19; COVID-19 - epidemiology; Disease control; Disease prevention; Distribution; Drug resistance; Drug resistance in microorganisms; Drug Resistance, Multiple, Bacterial - genetics; Epidemics; Epidemiology; Fatalities; Female; Health aspects; Health care facilities; Humans; Infant; Infections; Jurisdiction; Male; Medical diagnosis; Melting curve; Middle Aged; Mortality; Multidrug resistance; Mycobacterium tuberculosis; Mycobacterium tuberculosis - drug effects; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - isolation & purification; Mycobacterium tuberculosis complex; Pandemics; Patients; Population; Public health; Quality control; Quality standards; Regional differences; RpoB protein; SARS-CoV-2 - drug effects; SARS-CoV-2 - genetics; Sputum; Sputum - microbiology; Tuberculosis; Tuberculosis, Multidrug-Resistant - drug therapy; Tuberculosis, Multidrug-Resistant - epidemiology; Tuberculosis, Multidrug-Resistant - microbiology; Urban areas; Young Adult; China; Mycobacterium tuberculosis complex; Tuberculosis; Multidrug resistance; Regional differences
• ISSN: 1471-2334; 1471-2334
• DOI: 10.1186/s12879-024-09395-w

Tuberculosis (TB) remains a global public health event of great concern, however epidemic data on TB covering entire areas during the special period of the COVID-19 epidemic have rarely been reported. We compared the dissemination and multidrug-resistance patterns of Mycobacterium tuberculosis complex (MTBC) in the main urban area of Luoyang City, China (including six municipal jurisdictions) and nine county and township areas under its jurisdiction, aimed to establish the epidemiology of TB in this region and to provide reference for precision anti-TB in places with similar settings. From 2020 to 2022, sputum samples were collected from 18,504 patients with confirmed, suspected and unexcluded TB in 10 designated TB medical institutions. Insertion sequence 6110 was amplified by PCR (rpoB gene detection if necessary) to confirm the presence of MTBC. PCR-positive specimens were analyzed by multicolor melting curve analysis to detect multidrug resistance. Among the 18,504 specimens, 2675 (14.5%) were MTBC positive. The positive rate was higher in the main urban area than in the county and township areas (29.8% vs. 10.9%, p < 0.001). Male, re-treated and smear-positive groups were high-burden carriers of MTBC. Individuals aged > 60 years were the largest group infected with MTBC in the main urban area, compared with individuals aged < 61 years in the county and township areas. The detection of multidrug-resistant TB (MDR-TB) was higher in the main urban area than in the county and township areas (13.9% vs. 7.8%, p < 0.001). In all areas, MDR-TB groups were dominated by males, patients with a history of TB treatment, and patients aged < 61 years. Stratified analysis of MDR-TB epidemiology showed that MDR4 (INH þ RIF þ EMB þ SM) was predominant in the main urban area, while MDR3 (INH þ RIF þ SM) was predominant in the county and township areas. MDR-TB detection rate and epidemiology differed among the county and township areas. For local TB control, it is necessary to plan more appropriate and accurate prevention and control strategies according to the regional distribution of MTBC infection.