Incorporation of Caprylic Acid into a Single Cell Oil Rich in Docosahexaenoic Acid for the Production of Specialty Lipids

• Published in: Food technology and biotechnology Vol. 58; no. 4; pp. 411 - 422
• Main Authors: Kanno Mathias, Daniela, Piazentin Costa, Jacqueline, Rodrigues Calvo, Carolina, Claro da Silva, Roberta, Converti, Attilio, Segura, Nadia, Jachmanián, Iván, Gioielli, Luiz Antonio, Neves Rodrigues Ract, Juliana
• Format: Journal Article
• Language: English
• Published: Croatia Sveuciliste U Zagrebu 01.12.2020; Sveuciliste u Zagrebu, Prehramheno-Biotehnoloski Fakultet; University of Zagreb Faculty of Food Technology and Biotechnology
• Subjects: acidolysis; caprylic acid; Catalysts; Chemical reactions; Diet; Dietary supplements; Docosahexaenoic acid; Fatty acids; Lipase; Lipids; new source of docosahexaenoic acid; Octanoic acid; Oil; Oils & fats; Omega-3 fatty acids; Original Scientific Papers; Pharmaceutical industry; Saturated fatty acids; single cell oil; structured lipids; Substrates; Transesterification; Triglycerides; Unsaturated fatty acids; Yeast; Brazil; United States; caprylic acid; single cell oil; structured lipids; acidolysis; new source of docosahexaenoic acid
• ISSN: 1330-9862; 1334-2606
• DOI: 10.17113/ftb.58.04.20.6546

New sources of docosahexaenoic acid have recently been investigated aiming at infant formula fortification and dietary supplementation, among which the single cell oil with 40-50% of this acid. For this purpose, such an oil was blended with caprylic acid in amount substance ratio ranging from 1:1 to 5:1 and the blends were interesterified using either Novozym 435 or Lipozyme TL IM as the catalyst. The influence of the amount of excess free caprylic acid in the substrate, as well as the type of enzyme on the triacylglycerol rearrangement resulting from the synthesis of the structured lipids were evaluated. The regiospecific lipase Lipozyme TL IM seemed to induce transesterification among single cell oil triacylglycerols preferably by acidolysis with caprylic acid, which was directly proportional to the ratio of this acid in the substrate. In reactions catalyzed by the non-regiospecific lipase Novozym 435, a higher incorporation of caprylic acid into single cell oil triacylglycerols was observed than when using Lipozyme TL IM, independently of the oil/caprylic acid molar ratio. These results revealed the importance of combining the choice of the type of lipase, either regiospecific or not, with the amount ratios of free fatty acids and the substrate in acidolysis when aiming to produce structured lipids as a source of docosahexaenoic acid.