Long noncoding RNA NONHSAT122636.2 attenuates myocardial inflammation and apoptosis in myocarditis

• Published in: PloS one Vol. 19; no. 8; p. e0307779
• Main Authors: Liu, Yongjiao, Zhang, Li, Jia, Hailin, Feng, Xinxin, Ma, Mengjie, Wang, Jing, Han, Bo
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16.08.2024; Public Library of Science (PLoS)
• Subjects: Antisense RNA; Apoptosis; Apoptosis - drug effects; Assaying; Binding; Biomarkers; Blood; Cardiology; Cardiomyocytes; Cardiomyopathy; Cell viability; Cells; Child; Child, Preschool; Cyclin-dependent kinases; Cytokines; Cytokines - genetics; Cytokines - metabolism; Diagnostic systems; DNA-directed RNA polymerase; Enzyme-linked immunosorbent assay; Enzymes; Female; Flow cytometry; Gene expression; Guardians; Heart; Heart diseases; Humans; Infections; Inflammation; Inflammation - genetics; Inflammation - pathology; Injuries; Kinases; Leukocytes; Lipopolysaccharides; Lipopolysaccharides - pharmacology; Magnetic resonance imaging; Male; MicroRNA; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Myocarditis; Myocarditis - genetics; Myocarditis - metabolism; Myocarditis - pathology; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - metabolism; Myocytes, Cardiac - pathology; Non-coding RNA; Pediatrics; Peripheral blood; Physiological aspects; Polymerase chain reaction; Ribonucleic acid; RNA; RNA, Long Noncoding - genetics; Tumor necrosis factor-TNF; Viral infections; China; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0307779

The main pathological change of myocarditis is an inflammatory injury of cardiomyocytes. Long noncoding RNAs (lncRNAs) are closely related to inflammation, and our previous study showed that differential expression of lncRNAs is associated with myocarditis. This study aimed to investigate the impact of lncRNAs on the onset of myocarditis. RNA expression was measured by quantitative reverse-transcription polymerase chain reaction (RT-qPCR). Lipopolysaccharide (LPS) was used to induce inflammation in human cardiomyocytes (HCMs). The expression of inflammatory cytokines and myocardial injury markers was detected by enzyme-linked immunosorbent assay (ELISA) and RT-qPCR. Cell viability and apoptosis were measured by the cell counting kit-8 assay and flow cytometry. The binding force between lncRNA NONHSAT122636.2 and microRNA miRNA-2110 was detected using the dual-luciferase assay. NONHSAT122636.2 was dynamically expressed in patients with myocarditis and negatively correlated with inflammation severity. The overexpression of NONHSAT122636.2 improved inflammatory injury in LPS-stimulated HCMs. The study observed that there was a weak binding force between NONHSAT122636.2 and miR-2110. NONHSAT122636.2 attenuates myocardial inflammation and apoptosis in myocarditis. Additionally, its expression decreases in the peripheral blood of children suffering from myocarditis and in patients who are diagnosed for the first time showing higher diagnostic sensitivity and specificity. This decrease is negatively correlated with the degree of inflammation. Overall, the study suggests that NONHSAT122636.2 can be exploited as a potential diagnostic biomarker for pediatric myocarditis.