TaWRKY40 transcription factor positively regulate the expression of TaGAPC1 to enhance drought tolerance

• Published in: BMC genomics Vol. 20; no. 1; p. 795
• Main Authors: Zhang, Lin, Xu, Zhiyong, Ji, Haikun, Zhou, Ye, Yang, Shushen
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 30.10.2019; BioMed Central; BMC
• Subjects: Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis thaliana; Crop yields; Drought; Droughts; Enzymes; Gene Expression Regulation, Plant; Genetic aspects; Genetic engineering; Genetic research; Libraries; Luciferase; Monosaccharides; Phosphates; Physiological aspects; Plant hardiness; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - metabolism; Promoter Regions, Genetic; Signal Transduction; Stress, Physiological; TaGAPC1; TaWRKY40; Trans-Activators - metabolism; Transcription factors; Transcriptional Activation; Triticum - genetics; Triticum aestivum; Wheat; China; TaWRKY40; Drought; Triticum aestivum; TaGAPC1
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-019-6178-z

Drought stress is one of the major factors that affects wheat yield. Glyceraldehyde-3-Phosphate dehydrogenase (GAPDH) is a multifunctional enzyme that plays the important role in abiotic stress and plant development. However, in wheat, limited information about drought-responsive GAPC genes has been reported, and the mechanism underlying the regulation of the GAPC protein is unknown. In this study, we evaluated the potential role of GAPC1 in drought stress in wheat and Arabidopsis. We found that the overexpression of TaGAPC1 could enhance the tolerance to drought stress in transgenic Arabidopsis. Yeast one-hybrid library screening and EMSA showed that TaWRKY40 acts as a direct regulator of the TaGAPC1 gene. A dual luciferase reporter assay indicated that TaWRKY40 improved the TaGAPC1 promoter activity. The results of qRT-PCR in wheat protoplast cells with instantaneous overexpression of TaWRKY40 indicated that the expression level of TaGAPC1 induced by abiotic stress was upregulated by TaWRKY40. Moreover, TaGAPC1 promoted H O detoxification in response to drought. These results demonstrate that the inducible transcription factor TaWRKY40 could activate the transcription of the TaGAPC1 gene, thereby increasing the tolerance of plants to drought stress.