Rattlesnake Crotalphine Analgesic Active on Tetrodotoxin-Sensitive Na + Current in Mouse Dorsal Root Ganglion Neurons

• Published in: Toxins Vol. 16; no. 8; pp. 359 - 14
• Main Authors: Antunes, Aurélie, Robin, Philippe, Mourier, Gilles, Béroud, Rémy, De Waard, Michel, Servent, Denis, Benoit, Evelyne
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15.08.2024; MDPI
• Subjects: Analgesics; Analgesics - pharmacology; Animals; Cell Survival - drug effects; Cell viability; cell viability assay; Cells, Cultured; Crotalid Venoms - pharmacology; Crotalid Venoms - toxicity; crotalphine; Crotalus; Dorsal root ganglia; electrophysiology; Ganglia, Spinal - cytology; Ganglia, Spinal - drug effects; Hyperalgesia; Hypotheses; Inactivation; Iodides; Kinetics; Leakage current; Life Sciences; Male; Marine toxins; Membrane potential; Membrane Potentials - drug effects; Membranes; Mice; mouse dorsal root ganglion neurons; Narcotics; NAV1.7 Voltage-Gated Sodium Channel - metabolism; Neurobiology; Neurons; Neurons - drug effects; Neurons and Cognition; Pain; Pain perception; Peptides; Propidium iodide; rattlesnake toxin; Rattlesnakes; Sensitivity analysis; Snakes; Sodium Channel Blockers - pharmacology; Sodium channels (voltage-gated); Tetrodotoxin; Tetrodotoxin - pharmacology; tetrodotoxin-sensitive Na+ channels; Venom; France; tetrodotoxin-sensitive Na+ channels; mouse dorsal root ganglion neurons; rattlesnake toxin; electrophysiology; crotalphine; cell viability assay
• ISSN: 2072-6651; 2072-6651
• DOI: 10.3390/toxins16080359

Crotalphine is an analgesic peptide identified from the venom of the South American rattlesnake . Although its antinociceptive effect is well documented, its direct mechanisms of action are still unclear. The aim of the present work was to study the action of the crotalid peptide on the Na 1.7 channel subtype, a genetically validated pain target. To this purpose, the effects of crotalphine were evaluated on the Na 1.7 component of the tetrodotoxin-sensitive Na current in the dorsal root ganglion neurons of adult mice, using the whole-cell patch-clamp configuration, and on cell viability, using propidium iodide fluorescence and trypan blue assays. The results show that 18.7 µM of peptide inhibited 50% of the Na current. The blocking effect occurred without any marked change in the current activation and inactivation kinetics, but it was more important as the membrane potential was more positive. In addition, crotalphine induced an increase in the leakage current amplitude of approximately 150% and led to a maximal 31% decrease in cell viability at a high 50 µM concentration. Taken together, these results point out, for the first time, the effectiveness of crotalphine in acting on the Na 1.7 channel subtype, which may be an additional target contributing to the peptide analgesic properties and, also, although less efficiently, on a second cell plasma membrane component, leading to cell loss.