Tumor-specific enhanced NIR-II photoacoustic imaging via photothermal and low-pH coactivated AuNR@PNIPAM-VAA nanogel

• Published in: Journal of nanobiotechnology Vol. 22; no. 1; pp. 326 - 14
• Main Authors: Sun, Xiaodong, Li, Yujie, Liu, Xiaowan, Cui, Dandan, Shi, Yujiao, Huang, Guojia
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 10.06.2024; BioMed Central; BMC
• Subjects: Acetic acid; Acrylic Resins - chemistry; Animals; Cancer; Cell Line, Tumor; Chromophores; Diagnosis; Female; Gold - chemistry; Health aspects; Humans; Hydrogen-Ion Concentration; Infrared Rays; Medical imaging equipment; Mice; Mice, Inbred BALB C; Mice, Nude; Nanogel; Nanogels - chemistry; Nanotechnology; Nanotubes - chemistry; Near infrared spectroscopy; Neoplasms - diagnostic imaging; NIR-II; Organic acids; Photoacoustic imaging; phase transition; Photoacoustic Techniques - methods; Polyethylene Glycols - chemistry; Polyethyleneimine - chemistry; Technology application; Tumor detection; Tumor Microenvironment; Tumors; China; Iran; NIR-II; Nanogel; Photoacoustic imaging; phase transition; Tumor detection
• ISSN: 1477-3155; 1477-3155
• DOI: 10.1186/s12951-024-02617-y

Properly designed second near-infrared (NIR-II) nanoplatform that is responsive tumor microenvironment can intelligently distinguish between normal and cancerous tissues to achieve better targeting efficiency. Conventional photoacoustic nanoprobes are always "on", and tumor microenvironment-responsive nanoprobe can minimize the influence of endogenous chromophore background signals. Therefore, the development of nanoprobe that can respond to internal tumor microenvironment and external stimulus shows great application potential for the photoacoustic diagnosis of tumor. In this work, a low-pH-triggered thermal-responsive volume phase transition nanogel gold nanorod@poly(n-isopropylacrylamide)-vinyl acetic acid (AuNR@PNIPAM-VAA) was constructed for photoacoustic detection of tumor. Via an external near-infrared photothermal switch, the absorption of AuNR@PNIPAM-VAA nanogel in the tumor microenvironment can be dynamically regulated, so that AuNR@PNIPAM-VAA nanogel produces switchable photoacoustic signals in the NIR-II window for tumor-specific enhanced photoacoustic imaging. In vitro results show that at pH 5.8, the absorption and photoacoustic signal amplitude of AuNR@PNIPAM-VAA nanogel in NIR-II increases up obviously after photothermal modulating, while they remain slightly change at pH 7.4. Quantitative calculation presents that photoacoustic signal amplitude of AuNR@PNIPAM-VAA nanogel at 1064 nm has ~ 1.6 folds enhancement as temperature increases from 37.5 °C to 45 °C in simulative tumor microenvironment. In vivo results show that the prepared AuNR@PNIPAM-VAA nanogel can achieve enhanced NIR-II photoacoustic imaging for selective tumor detection through dynamically responding to thermal field, which can be precisely controlled by external light. This work will offer a viable strategy for the tumor-specific photoacoustic imaging using NIR light to regulate the thermal field and target the low pH tumor microenvironment, which is expected to realize accurate and dynamic monitoring of tumor diagnosis and treatment.