Comparative Mitogenomic Analyses of Tanypodinae (Diptera: Chironomidae)

• Published in: Insects (Basel, Switzerland) Vol. 16; no. 2; p. 203
• Main Authors: Xiao, Xiu-Ru, Chen, Meng-Han, Li, Shu-Yi, Guo, Bing-Xin, Zhang, Yan, Zhang, Zhi-Chao, Qiao, Ya-Jun, Lin, Xiao-Long
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.02.2025; MDPI
• Subjects: Bayesian analysis; characteristics; Chironomidae; Chironomids; Cladistic analysis; Comparative analysis; Genes; Genetic aspects; Genomes; Genomic analysis; Genomics; Identification and classification; Mitochondrial DNA; mitogenome; Morphology; Phylogenetics; Phylogeny; Physiological aspects; Statistical inference; Tanypodinae; Taxonomy; Transfer RNA; Zoology; China; United States > US; Germany; Spain; phylogeny; characteristics; mitogenome
• ISSN: 2075-4450; 2075-4450
• DOI: 10.3390/insects16020203

Tanypodinae, a highly diverse subfamily within Chironomidae (Diptera), has been poorly represented in mitochondrial genome (mitogenome) databases, with only a limited number of complete or partial sequences available. To address this gap, we present the first complete mitogenome sequences of 16 Tanypodinae species and 1 Podonominae species. By integrating these novel data with previously published chironomid mitogenomes, we conducted a comprehensive comparative mitogenomic analysis of 21 Tanypodinae species. Our results reveal that Tanypodinae mitogenomes maintain a conserved structural organization, preserving the ancestral insect gene arrangement. The nucleotide composition exhibits significant bias, with the control region demonstrating the highest A + T content among all genomic regions. Evolutionary analysis indicates that all protein-coding genes (PCGs) are subject to purifying selection, with exhibiting the most rapid evolutionary rate. Phylogenetic reconstruction based on PCG amino acid sequences delineates seven well-supported clades within Tanypodinae, corresponding to the tribes Pentaneurini, Natarsiini, Procladiini, Tanypodini, Clinotanypodini, Macropelopiini, and Anatopyniini. These findings significantly advance our understanding of mitogenomic architecture and provide critical insights into the phylogenetic relationships within Tanypodinae.