Indoleamine 2,3-Dioxygenase Is Dispensable for The Immunomodulatory Function of Stem Cells from Human Exfoliated Deciduous Teeth

• Published in: Cell journal (Yakhteh) Vol. 18; no. 4; pp. 597 - 608
• Main Authors: Alipour, Razieh, Masoumi Karimi, Masoumeh, Hashemi-Beni, Batool, Adib, Minoo, Sereshki, Nasrin, Sadeghi, Farzaneh
• Format: Journal Article
• Language: English
• Published: Iran Royan Institute of Iran 2017; Royan Institute; Royan Institute (ACECR), Tehran
• Subjects: Accessibility; Adipose tissue; Age; Amniotic membrane; Angiogenesis; Animal models; Antibodies; Approximation; Arches; Assaying; Biocompatibility; Blood cells; Blood circulation; Bone marrow; Cancer; Catalysts; CD3 antigen; CD4 antigen; CD8 antigen; Cell activation; Cell culture; Conditioning; Cytokines; Cytotoxicity; Degradation; Dendritic cells; Differentiation; Effectors; Engineering; Enzymes; Growth factors; Hypersensitivity; IDO; IFN-γ; Immune system; Immunomodulation; Immunoregulation; Immunosuppression; In vivo methods and tests; Inflammation; Inhibition; Interferon; Leukocytes (mononuclear); Lymphocytes; Lymphocytes B; Lymphocytes T; Mathematical analysis; Mesenchymal stem cells; Mesenchymal Stem/Stromal Cells; Metabolites; Neutralization; Organs; Original; Pain; Peripheral blood mononuclear cells; Placenta; Polarization; Properties (attributes); Prostaglandins; SHED; Stem cells; Stimulation; Studies; Teeth; Tissues; Toxicity; Tryptophan; Tryptophan 2,3-dioxygenase; Wound healing; γ-Interferon; IFN-γ; SHED; IDO; Immunomodulation; Mesenchymal Stem/Stromal Cells
• ISSN: 2228-5806; 2228-5814
• DOI: 10.22074/cellj.2016.4726

In this study, we sought to better understand the immunoregulatory function of stem cells derived from human exfoliated deciduous teeth (SHED). We studied the role of the interferon gamma (IFN-γ)-indoleamine 2,3-dioxygenase (IDO)-axis in immunoregulation of SHED compared to bone marrow derived mesenchymal stem cells (BMMSCs) under the same conditions. In this cross-sectional study, recently isolated human T cells were stimulated either by mitogen or inactivated allogeneic peripheral blood mononuclear cells (PBMCs). These T cells were subsequently co-cultured with, either SHED or BMMSCs in the presence or absence of 1-methyl-tryptophan (1-MT) or neutralizing anti- human-IFN-γ antibodies. In all co-cultures we evaluated lymphocyte activation as well as IDO activity. SHED, similar to conventional BMMSCs, had anti-proliferative effects on stimulated T cells and reduced their cytokine production. This property of SHED and BMMSCs was changed by IFN-γ neutralization. We detected IDO in the immunosuppressive supernatant of all co-cultures. Removal of IDO decreased the immunosuppression of BMMSCs. SHED, like BMMSCs, produced the IDO enzyme. Although IFN-γ is one of inducer of IDO production in SHED, these cells were not affected by IFN-γ in the same manner as BMMSCs. Unlike BMMSCs, the IDO enzyme did not contribute to their immunosuppression and might have other cell-type specific roles.