Purification and characterization of a novel type of neurotoxic peptides from the venom of the Iranian scorpion Hemiscorpius lepturus

Scorpion venom has toxic effects on mammals, insects and crustaceans. Toxicogenic peptides are major contributors to the scorpion venom, which make it toxic. The is one of the most common scorpion bites agent, and responsible for 95% of scorpion bite deaths cases in Iran. In this project, we fractio...

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Bibliographic Details
Published inIranian journal of basic medical sciences Vol. 23; no. 2; pp. 195 - 201
Main Authors Maleki, Mahboob, Mohammadpour Dounighi, Naser
Format Journal Article
LanguageEnglish
Published Iran Mashhad University of Medical Sciences 01.02.2020
Subjects
Chromatography
hemiscorpius lepturus
Molecular weight
Original
Peptides
purification
scorpion venom
toxic peptides
toxin
Venom
Toxin
Hemiscorpius lepturus
Toxic peptides
Purification
Scorpion venom
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Summary:Scorpion venom has toxic effects on mammals, insects and crustaceans. Toxicogenic peptides are major contributors to the scorpion venom, which make it toxic. The is one of the most common scorpion bites agent, and responsible for 95% of scorpion bite deaths cases in Iran. In this project, we fractionated the scorpion venom and analyzed toxic fractions of the venom. The crude venom of was dialyzed against distilled water and then the soluble part of the venom was isolated from the non-soluble (mucoproteins) part of the venom and loaded onto the Sephadex G-50 gel filtration column, then after determining the toxicity of the obtained fractions (fractions toxicity were detected in mice by IV injection), the resulting toxic fraction was purified with three stages of ion-exchange chromatography (anion and cationic) and RP-HPLC. The purity of the fractions was verified by SDS-PAGE electrophoreses. The LD of venom was 177.01 µg/mouse. The crude venom had 7 detectable bands with molecular weights of 10-100 KDa and one band less than 10 KDa. Finally, after the different stages of chromatography, two HL2153 and HL2155 peaks were obtained from the RP-HPLC, which were depicted single bands and high purity. The electrophoretic analysis showed molecular weight 4874 Da for HL2153 and 5107 Da for HL2155 toxins. It is concluded that venom contains two HL2153 and HL2155 toxins with a relatively similar molecular weight and similar electrical charge 4874 and 5107 Da, respectively.
ISSN:2008-3866
2008-3874
DOI:10.22038/IJBMS.2019.37910.9025