Anti-MRSA activity of a bioactive compound produced by a marine Streptomyces and its optimization using statistical experimental design

To address the alarming problem of methicillin-resistant (MRSA), herein, a marine capable of producing an anti-MRSA compound has been studied. Strain MN41 was morphologically and physiologically characterized and then, molecularly identified using 16SrRNA analysis. To produce the bioactive compound...

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Published inIranian journal of basic medical sciences Vol. 22; no. 9; pp. 1073 - 1084
Main Authors Norouzi, Hamed, Rabbani Khorasgani, Mohammad, Danesh, Abolghasem
Format Journal Article
LanguageEnglish
Published Iran Mashhad University of Medical Sciences 01.09.2019
Subjects
Experimental designs
Fermentation
Marine microbiology
Methicillin-Resistant Staphylococcus aureus
Natural products
NMR
Nuclear magnetic resonance
Optimization
Original
Staphylococcus infections
Methicillin-resistant - Staphylococcus aureus
Experimental designs
Natural products Fermentation
Marine microbiology
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Summary:To address the alarming problem of methicillin-resistant (MRSA), herein, a marine capable of producing an anti-MRSA compound has been studied. Strain MN41 was morphologically and physiologically characterized and then, molecularly identified using 16SrRNA analysis. To produce the bioactive compound in large scale, a kind of submerged liquid fermentation was adopted. The antibacterial agent was purified using a silica gel column followed by a semi-preparative HPLC and the isolated metabolite was identified using mass spectrometry, Nuclear magnetic resonance (NMR) and Fourier-transform infrared (FTIR). Finally, the production process was subjected to a two steps optimization using Plackett-Burman design (PBD) and Response Surface Method (RSM), respectively. In addition, the antitumor activity of the active agent was studied. The purified compound with a molecular weight of 421.2 was identified as a natural pyrrole-derivative. The optimization revealed a significant effect for starch, pH, calcium carbonate and peptone on the production of this anti-MRSA compound and resulted in a 218% increase in the production yield. The isolated pyrrole-derivative showed a remarkable activity against MRSA and also showed some promising anti-tumor activity.
Bibliography:ObjectType-Article-1
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ISSN:2008-3866
2008-3874
DOI:10.22038/IJBMS.2019.33880.8058