Elucidation of flavonoids from potent Iranian Scutellaria species against Influenza A (H1N1) virus

• Published in: Iranian journal of basic medical sciences Vol. 26; no. 1; pp. 76 - 84
• Main Authors: Pirali Hamedani, Mostafa, Ahmad Kashi, Mahtab, Goodarzi, Saied, Shokouhi, Hadiseh, Shafiee Ardestani, Mahdi, Hadjiakhoondi, Abbas, Pirali Hamedani, Morteza, Ghahremani, Mohammad Hosein, Mehrbod, Parvaneh, Tofighi, Zahra
• Format: Journal Article
• Language: English
• Published: Iran Mashhad University of Medical Sciences 01.01.2023
• Subjects: antiviral; Cytotoxicity; Flavonoids; folin-ciocalteau reagent; h1n1 virus; Influenza; mtt assay; Original; scutellaria; Antiviral; Flavonoids; Folin-ciocalteau reagent; Scutellaria; Influenza A virus; MTT assay
• ISSN: 2008-3866; 2008-3874
• DOI: 10.22038/IJBMS.2022.66255.14553

Influenza A virus (IAV) is a contagious illness. Different species of genus are used as a traditional remedy to reduce influenza symptoms. This study aimed to investigate the anti-influenza capacity of several species of Iranian Scutellaria and identify active compounds of the most potent species for the first time. Some Iranian species of were collected from different regions of Iran, including with , , and subspecies; ; ; . They were fractionated to chloroform and methanol. The total phenols and flavonoids of samples were examined by the folin-ciocalteau and aluminum-flavonoid complex methods, respectively. The 50% cytotoxic concentrations (CC ) on MDCK cells and non-cytotoxic concentrations (NCTC) were determined by MTT assay. The percentage of cell protection against IAV and their effect on virus titer were investigated in pre-, post-, and co-penetration treatment groups. Phytochemicals of the most effective species were isolated by various chromatographic methods and identified by different spectroscopic methods. Methanol fraction of subsp. demonstrated the highest amounts of flavonoid content and best activity against influenza A virus in all combination treatments, which reduced the virus titer by 5 logs with no cytotoxicity. Kaempferol-3- -glucoside, quercetin-3- -glucoside, apigenin-4'-methoxy-7- -glucoside, luteolin, and luteolin-7- -glucoside were purified and identified from this species. subsp. can be introduced as a source of flavonoids with acceptable anti-influenza activity. also showed potent antiviral effects and is a candidate for elucidation in further studies.