Histone Modifications of H3K4me3, H3K9me3 and Lineage Gene Expressions in Chimeric Mouse Embryo

• Published in: Cell journal (Yakhteh) Vol. 22; no. 1; pp. 96 - 105
• Main Authors: Salimi, Maryam, Shirazi, Abolfazl, Norouzian, Mohsen, Mehrazar, Mohammad Mehdi, Naderi, Mohammad Mehdi, Shokrgozar, Mohammad Ali, Omrani, Mirdavood, Hashemi, Seyed Mahmoud
• Format: Journal Article
• Language: English
• Published: Iran Royan Institute of Iran 01.04.2020; Royan Institute; Royan Institute (ACECR), Tehran
• Subjects: Abnormalities; Amino acids; Blastocysts; CDX2 protein; Cell culture; Cell fate; Cell lineage; Cell Lineage Genes; Chimera; Deoxyribonucleic acid; DNA; DNA methylation; Embryo cells; Embryos; Epigenetics; Females; Fetuses; Gene expression; Genes; Genetic engineering; Glycerol; H3Methylation; Histones; In vitro fertilization; Injection; Oct-4 protein; Original; Placenta; Pluripotency; Polyvinyl alcohol; Stem cell transplantation; Stem cells; Studies; Trophectoderm; Viability; Iran; United States > US; Japan; Cell Lineage Genes; H3Methylation; Chimera
• ISSN: 2228-5806; 2228-5814
• DOI: 10.22074/cellj.2020.6443

Chimeric animal exhibits less viability and more fetal and placental abnormalities than normal animal. This study was aimed to determine the impact of mouse embryonic stem cells (mESCs) injection into the mouse embryos on H3K9me3 and H3K4me3 and cell lineage gene expressions in chimeric blastocysts. In our experiment, at the first step, incorporation of the GFP positive mESCs (GFP-mESCs) 129/Sv into the inner cell mass (ICM) of pre-compacted and compacted morula stage embryos was compared. At the second and third steps, H3K4me3 and H3K9me3 status as well as the expression of genes were determined in the following groups: i. blastocyst derived from morula subjected to mESCs injection (blast/chimeric), ii. derived blastocyst (blast/ ), iii. blastocyst derived from culture of morula (blast/morula), and iv. blastocyst derived from morula subjected to sham injection (blast/sham). Subzonal injection of GFP-mESCs at the pre-compacted embryos produced more chimeric blastocysts than compacted embryos (P<0.05). The number of trophectoderm (TE), ICM, ICM/TE and total cells in chimeric blastocysts were less than the corresponding numbers in blastocysts derived from other groups (P<0.05). In ICM and TE of chimeric blastocysts, the levels of H3K4me3 and H3K9me3 were respectively decreased and increased compared to the blastocysts of the other groups (P<0.05). Expressions of were decreased in chimeric blastocysts compared to the blastocysts of the other groups (P<0.05), while this was not observed for . In the present study, embryo compaction significantly reduced the rate of incorporation of injected mESCs into the ICM. Moreover, in chimeric blastocysts, the levels of H3K9me3 and H3K4me3 were altered. In addition, the expressions of pluripotency and cell fate genes were decreased compared to blastocysts of the other groups.