A long-term prospecting study on giant viruses in terrestrial and marine Brazilian biomes

The discovery of mimivirus in 2003 prompted the search for novel giant viruses worldwide. Despite increasing interest, the diversity and distribution of giant viruses is barely known. Here, we present data from a 2012-2022 study aimed at prospecting for amoebal viruses in water, soil, mud, and sewag...

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Published inVirology journal Vol. 21; no. 1; pp. 135 - 11
Main Authors Machado, Talita B, de Aquino, Isabella L. M, Azevedo, Bruna L, Serafim, Mateus S, Barcelos, Matheus G, Andrade, Ana Cláudia S. P, Reis, Erik, Ullmann, Leila Sabrina, Pessoa, João, Costa, Adriana O, Rosa, Luiz H, Abrahão, Jônatas S
Format Journal Article
LanguageEnglish
Published London BioMed Central Ltd 10.06.2024
BioMed Central
BMC
Subjects
Amoebas
Analysis
Biological diversity
Biomes
Distribution
Diversity
Environmental aspects
Giant virus
Richness
Virus research
Viruses
Brazil
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Summary:The discovery of mimivirus in 2003 prompted the search for novel giant viruses worldwide. Despite increasing interest, the diversity and distribution of giant viruses is barely known. Here, we present data from a 2012-2022 study aimed at prospecting for amoebal viruses in water, soil, mud, and sewage samples across Brazilian biomes, using Acanthamoeba castellanii for isolation. A total of 881 aliquots from 187 samples covering terrestrial and marine Brazilian biomes were processed. Electron microscopy and PCR were used to identify the obtained isolates. Sixty-seven amoebal viruses were isolated, including mimiviruses, marseilleviruses, pandoraviruses, cedratviruses, and yaraviruses. Viruses were isolated from all tested sample types and almost all biomes. In comparison to other similar studies, our work isolated a substantial number of Marseillevirus and cedratvirus representatives. Taken together, our results used a combination of isolation techniques with microscopy, PCR, and sequencing and put highlight on richness of giant virus present in different terrestrial and marine Brazilian biomes.
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ISSN:1743-422X
1743-422X
DOI:10.1186/s12985-024-02404-z