A Strain of an Emerging Indian Xanthomonas oryzae pv. oryzae Pathotype Defeats the Rice Bacterial Blight Resistance Gene xa13 Without Inducing a Clade III SWEET Gene and Is Nearly Identical to a Recent Thai Isolate

• Published in: Frontiers in microbiology Vol. 9; p. 2703
• Main Authors: Carpenter, Sara C D, Mishra, Prashant, Ghoshal, Chandrika, Dash, Prasanta K, Wang, Li, Midha, Samriti, Laha, Gouri S, Lore, Jagjeet S, Kositratana, Wichai, Singh, Nagendra K, Singh, Kuldeep, Patil, Prabhu B, Oliva, Ricardo, Patarapuwadol, Sujin, Bogdanove, Adam J, Rai, Rhitu
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 13.11.2018
• Subjects: bacterial blight of rice; Microbiology; SMRT sequencing; susceptibility genes; SWEET genes; transcription activator-like effectors (TALEs); SMRT sequencing; SWEET genes; bacterial blight of rice; susceptibility genes; transcription activator-like effectors (TALEs)
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2018.02703

The rice bacterial blight pathogen pv. oryzae ( ) injects transcription activator-like effectors (TALEs) that bind and activate host "susceptibility" ( ) genes important for disease. Clade III genes are major S genes for bacterial blight. The resistance genes , which reduces TALE activity generally, and , a allele not recognized by the cognate TALE, have been effectively deployed. However, strains that defeat both resistance genes individually were recently reported in India and Thailand. To gain insight into the mechanism(s), we completely sequenced the genome of one such strain from each country and examined the encoded TALEs. Strikingly, the two strains are clones, sharing nearly identical TALE repertoires, including a TALE known to activate strongly enough to be effective even when diminished by . We next investigated gene induction by the Indian strain. The Indian strain induced no clade III in plants harboring , indicating a pathogen adaptation that relieves dependence on these genes for susceptibility. The findings open a door to mechanistic understanding of the role genes play in susceptibility and illustrate the importance of complete genome sequence-based monitoring of populations in developing varieties with effective disease resistance.