The Impact of Protein Glycosylation on the Identification of Patients with Pediatric Appendicitis

The identification of pediatric appendicitis is challenging due to the lack of specific markers thereby several factors are included in the diagnostic process such as abdominal pain, ultrasonography and altered laboratory parameters (C reactive protein, absolute neutrophil cell number and white bloo...

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Published inInternational journal of molecular sciences Vol. 25; no. 12; p. 6432
Main Authors Dojcsák, Dalma, Farkas, Flóra, Farkas, Tamás, Papp, János, Garami, Attila, Viskolcz, Béla, Váradi, Csaba
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.06.2024
MDPI
Subjects
Abdomen
Appendicitis
Blood cell count
C-reactive protein
Enzymes
Liquid chromatography
mass spectrometry
Medical research
Medicine, Experimental
Normal distribution
Patients
Pediatrics
Procainamide
Proteins
serum glycosylation
Statistical analysis
Hungary
Taiwan
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Summary:The identification of pediatric appendicitis is challenging due to the lack of specific markers thereby several factors are included in the diagnostic process such as abdominal pain, ultrasonography and altered laboratory parameters (C reactive protein, absolute neutrophil cell number and white blood cell number). The glycosylation pattern of serum N-glycome was analyzed in this study of 38 controls and 40 patients with pediatric appendicitis. The glycans were released by enzymatic deglycosylation followed by fluorescent labeling and solid-phase extraction. The prepared samples were analyzed by hydrophilic interaction liquid chromatography with fluorescence and mass-spectrometric detection. The generated data were analyzed by multiple statistical tests involving the most important laboratory parameters as well. Significant differences associated with the examined patient groups were revealed suggesting the potential use of glycosylation analysis supporting the detection of pediatric appendicitis.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25126432