A DNA vaccine encoding for TcSSP4 induces protection against acute and chronic infection in experimental Chagas disease

Immunization of mice with plasmids containing genes of Trypanosoma cruzi induces protective immunity in the murine model of Chagas disease. A cDNA clone that codes for an amastigote-specific surface protein (TcSSP4) was used as a candidate to develop a DNA vaccine. Mice were immunized with the recom...

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Published inInternational journal of biological sciences Vol. 7; no. 9; pp. 1230 - 1238
Main Authors Arce-Fonseca, Minerva, Ramos-Ligonio, Angel, López-Monteón, Aracely, Salgado-Jiménez, Berenice, Talamás-Rohana, Patricia, Rosales-Encina, José Luis
Format Journal Article
LanguageEnglish
Published Australia Ivyspring International Publisher 01.01.2011
Subjects
Animals
Chagas Disease - immunology
Chagas Disease - prevention & control
Cytokines - blood
Female
Interferon-gamma - metabolism
Mice
Mice, Inbred BALB C
Myocarditis - parasitology
Myocarditis - prevention & control
Plasmids - genetics
Protozoan Proteins
Research Paper
Th1 Cells - immunology
Trypanosoma cruzi - genetics
Trypanosoma cruzi - immunology
Trypanosoma cruzi - pathogenicity
Vaccines, DNA - immunology
Vaccines, DNA - therapeutic use
DNA immunization
TcSSP4
Trypanosoma cruzi
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Summary:Immunization of mice with plasmids containing genes of Trypanosoma cruzi induces protective immunity in the murine model of Chagas disease. A cDNA clone that codes for an amastigote-specific surface protein (TcSSP4) was used as a candidate to develop a DNA vaccine. Mice were immunized with the recombinant protein rTcSSP4 and with cDNA for TcSSP4, and challenged with bloodstream trypomastigotes. Immunization with rTcSSP4 protein makes mice more susceptible to trypomastigote infection, with high mortality rates, whereas mice immunized with a eukaryotic expression plasmid containing the TcSSP4 cDNA were able to control the acute phase of infection. Heart tissue of gene-vaccinated animals did not show myocarditis and tissue damage at 365 days following infection, as compared with control animals. INF-γ was detected in sera of DNA vaccinated mice shortly after immunization, suggesting the development of a Th1 response. The TcSSP4 gene is a promising candidate for the development of an anti-T. cruzi DNA vaccine.
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These authors contributed equally to this work.
Conflict of Interests: The authors have declared that no conflict of interest exists.
ISSN:1449-2288
1449-2288
DOI:10.7150/ijbs.7.1230