Post-Effort Changes in Activity of Traditional Diagnostic Enzymatic Markers in Football Players' Blood

• Published in: Journal of medical biochemistry Vol. 34; no. 2; pp. 179 - 190
• Main Authors: Chamera, Tomasz, Spieszny, Michał, Klocek, Tomasz, Kostrzewa-Nowak, Dorota, Nowak, Robert, Lachowicz, Milena, Buryta, Rafał, Ficek, Krzysztof, Eider, Jerzy, Moska, Waldemar, Cięszczyk, Paweł
• Format: Journal Article
• Language: English
• Published: Serbia Society of Medical Biochemists of Serbia 01.04.2015; Society of Medical Biochemists of Serbia, Belgrade
• Subjects: aerobic training; athletes; enzymatic markers; Original Paper; enzymatic markers; athletes; aerobic training
• ISSN: 1452-8258; 1452-8266
• DOI: 10.2478/jomb-2014-0035

Long-term and intensive physical effort causes metabolic and biochemical adaptations for both athletic and non-athletic objectives. Knowing the importance of aerobic training in football players, the aim of this study was to evaluate changes in the activity of: creatinine kinase (CK), creatine kinase MB (CKMB), lactate dehydrogenase (LDH), α-hydroxybutyrate dehydrogenase (HBDH), cholinesterase (ChE) and alkaline phosphatase (ALP) in response to a semi-long distance outdoor run under aerobic conditions among both female and male football players. Sixteen participants aged 21.9±2 years (women) and 18.4±0.5 years (men), all of them voluntarily recruited football players, took part in an outdoor run, the women covering a distance of 7.4±0.3 km while men covered a distance of 10.7±1.0 km. Plasma activities of the studied enzymes were determined using an appropriate diagnostic assay kit. Our results indicate that total LDH activity could be a useful tool in evaluating physical fitness among athletes. We simultaneously established that ChE could not be a marker useful in assessing metabolic response to physical effort in athletes. Moreover, our results suggest that post-effort changes in ALP activity might be used to estimate early symptoms of certain vitamin deficiencies in an athlete's diet. We confirmed that the assessment of activity of selected traditional diagnostic enzymatic markers provides information about muscle state after physical effort.