Histo-morphological and seminal evaluation of testicular parameters in diabetic rats under antiretroviral therapy: interactions with Hypoxis hemerocallidea

• Published in: Iranian journal of basic medical sciences Vol. 21; no. 12; pp. 1322 - 1330
• Main Authors: Olasile, Ismail Onanuga, Jegede, I Ayoola, Ugochukwu, Offor, Ogedengbe, O Oluwatosin, Naidu, Edwin Cs, Peter, I Aniekan, Azu, Onyemaechi O
• Format: Journal Article
• Language: English
• Published: Iran Mashhad University of Medical Sciences 01.12.2018
• Subjects: Antiretroviral drugs; Diabetes; Drug therapy; Experimental diabetes; HAART; Hypoxis hemerocallidea; Melatonin; Rodents; Seminal fluid; Short Communication; Sperm; Testis; Testis; Experimental diabetes; HAART; Hypoxis hemerocallidea Seminal fluid
• ISSN: 2008-3866; 2008-3874
• DOI: 10.22038/ijbms.2018.25046.6213

Broad range of metabolic changes associated with highly active antiretroviral therapy (HAART) has been reported over decades including reproductive perturbations. The current study aimed at investigating the role of in the seminal and morphometric alterations in the testes of streptozotocin-nicotinamide-induced diabetic rats under HAART. Sixty-two adult male Sprague-Dawley rats were divided into A-H groups, containing 6 rats in the control group A and 8 rats in the treatment groups B-H. Diabetes was induced by intraperitoneal injection of nicotinamide (110 mg/kg BW) followed by streptozotocin (45 mg/kg BW). The animals were then subjected to various treatments with HAART, Hyp, and melatonin. weights (body and testicular), histological, histochemical, seminal fluid, and morphometric analyses were carried out. Sperm count and motility were reduced in HAART ( 0.05/0.003) and Hyp200 ( .003) groups compared with normal and diabetic controls, respectively. Sperm count was higher ( .003) in HAART+ Mel and HAART+Hyp100 groups. Morphometry showed the reduction in germinal epithelium height and basement membrane thickness ( .003) in the Hyp100 group compared with diabetic controls. Adjuvant use of and melatonin with HAART did not significantly raise these indices ( .05). Histological slides showed gross distortions in HAART, diabetic and HAART +Hyp groups with marked atrophy in tubules, germ cell loss and areas of focal depletion of the cell. PAS staining revealed detached basement membrane in diabetic groups with strong PAS-stain. The use of or melatonin does not ameliorate the testicular damages in diabetic animals under antiretroviral therapy.