Detection of tetracycline resistance genes, aminoglycoside modifying enzymes, and coagulase gene typing of clinical isolates of Staphylococcus aureus in the Southwest of Iran

• Published in: Iranian journal of basic medical sciences Vol. 20; no. 8; pp. 912 - 919
• Main Authors: Khoramrooz, Seyed Sajjad, Dolatabad, Samin Alipoor, Dolatabad, Fatemeh Mostafapour, Marashifard, Masoud, Mirzaii, Mehdi, Dabiri, Hossein, Haddadi, Azam, Rabani, Seyed Mohammadreza, Shirazi, Hamid Reza Ghaffarian, Darban-Sarokhalil, Davood
• Format: Journal Article
• Language: English
• Published: Iran Mashhad University of Medical Sciences 01.08.2017
• Subjects: Aminoglycoside modifying enzymes; Aminoglycoside resistance; Antibiotics; Coagulase gene typing; Enzymes; Genes; Original; Penicillin; Staphylococcus aureus; Staphylococcus infections; Tetracycline resistance; Iran; Coagulase gene typing; Aminoglycoside resistance; Tetracycline resistance; Aminoglycoside modifying - enzymes; Staphylococcus aureus
• ISSN: 2008-3866; 2008-3874
• DOI: 10.22038/ijbms.2017.9114

The aim of the present study was to determine the aminoglycoside modifying enzymes (AMEs) encoded genes, tetracycline resistance genes, and the based typing of isolates in the Southwest of Iran. Antimicrobial susceptibility of isolates was carried out by agar disk diffusion methods. Two sets of multiplex PCR mixture were used for detection of AME genes and genes. All of the isolates were typed with the coagulase gene typing method. Of the 121 isolates, 29.75% and 47.93% were resistant to at least one aminoglycosides and tetracyclines, respectively. The was the most frequent gene (97.22%), and and genes were detected in 61.11% and 11.11% of aminoglycoside resistant isolates, respectively. The K and M genes were detected in 82.75% and 56.9% of tetracycline resistant isolates, respectively. Overall 31.4% of isolates were MRSA. Totally 17 distinct gene RFLP patterns, numbered C1 to C17, were observed. The C5 was the most frequent type with 31 isolates. The and genes were the most important genes contributing to aminoglycosides resistance, while resistance to tetracyclines was mediated by K and M genes. Interestingly all with C5 as the most prevalent -type were resistant to at least one of the aminoglycoside antibiotics and tetracycline simultaneously. Moreover, 30 out of 31 isolates with this type were MRSA, indicating the importance of the C5 -type in MRSA strains and also in isolates that were resistant to aminoglycosides and tetracycline.