Comparative Analysis of Immune Gene Transcription in Sea Bream ( Sparus aurata ) Challenged with RGNNV or RGNNV/SJNNV Betanodaviruses

• Published in: Pathogens (Basel) Vol. 13; no. 6; p. 478
• Main Authors: Gemez-Mata, Juan, Moreno, Patricia, Alvarez-Torres, Daniel, Garcia-Rosado, Esther, Bejar, Julia, Alonso, M Carmen
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04.06.2024; MDPI
• Subjects: Aquaculture; Bass; Brain; Bream; Comparative analysis; Cytokines; Disease resistance; European sea bream; Fish diseases; Genes; Genomes; Hsp70 protein; Immune response; Immune system; Infections; innate immune response; Mortality; Necrosis; Principal components analysis; Proteins; Replication; RGNNV; RGNNV/SJNNV; RNA polymerase; Sea bass; Transcription; Tumor necrosis factor-TNF; Viruses; United Kingdom > UK; United States > US; Germany; European sea bream; RGNNV/SJNNV; RGNNV; innate immune response
• ISSN: 2076-0817; 2076-0817
• DOI: 10.3390/pathogens13060478

Gilthead sea bream and European sea bass display different resistance-susceptibility patterns during infection with different nervous necrosis virus (NNV) species, which may derive from differences in the triggered immune response. Based on this premise, we analysed the transcription of several selected immune-related genes in sea bream experimentally infected with NNV isolates obtained from sea bass (DlNNV, RGNNV) or sea bream (SaNNV, RGNNV/SJNNV). Viral replication only occurred in SaNNV-inoculated fish; therefore, the differences between the immune response elicited by both viruses may be the key to understanding the mechanism behind the inhibition of DlNNV replication. Principal component analysis clustered samples according to the viral isolate from 1 day post infection onwards and evidenced differences in the immune response against both viruses, even though no mortalities or symptoms were recorded. The response against DlNNV is characterized by higher transcription early after the infection, longer-lasting transcription and stronger induction of and . These genes should be targets for future studies in order to elucidate their role in hampering NNV replication in sea bream, which is essential for developing effective prophylactic measures.