The Effect of Monophosphoryl Lipid A on Maturation of DCs from Patients with Acute Myeloid Leukaemia

• Published in: Iranian journal of immunology Vol. 14; no. 1; pp. 1 - 12
• Main Authors: El Husseiny, Noha Mohamed, El Fattah Attya Elkak, Walaa Abd, El Ansary, Mervat Said, El Aziz El Khateeb, Engy Mohamed Abd, Mattar, Mervat Wagih, El Demerdash, Doaa Mohamed
• Format: Journal Article
• Language: English
• Published: Iran Shiraz Institute for Cancer Research 01.03.2017; Shiraz University of Medical Sciences
• Subjects: Acute myeloid leukemia; Antigens; Antigens, Neoplasm - immunology; B7-2 Antigen - metabolism; Cancer therapies; CCR7 protein; CD83 antigen; CD86 antigen; Cell culture; Cell Differentiation - drug effects; Cells, Cultured; Cytokines; Cytotoxicity; DCs Maturation; Dendritic cells; Dendritic Cells - drug effects; Dendritic Cells - immunology; Dendritic Cells - transplantation; Gangrene; Granulocyte-macrophage colony-stimulating factor; Hematology; Humans; Immunotherapy, Adoptive - methods; Interleukin 10; Interleukin 4; Interleukin-1beta - immunology; Internal medicine; Leukemia; Leukemia, Myeloid, Acute - immunology; Leukemia, Myeloid, Acute - therapy; Ligands; Lipid A; Lipid A - analogs & derivatives; Lipid A - pharmacology; Lipids; Lung cancer; Maturation; Medicine; Monocyte-Derived DCs; Monocytes; Monophosphoryl lipid A; Peptide Fragments - immunology; Phenotypes; Receptors, CCR7 - metabolism; Remission; TLR4 protein; Toll-Like Receptor 4 - agonists; Toll-like receptors; Tumor necrosis factor; Tumor Necrosis Factor-alpha - immunology; Tumor necrosis factor-TNF; Tumor necrosis factor-α
• ISSN: 1735-1383; 1735-367X; 1735-367X

Generation of monocyte-derived dendritic cells (MDDC) is induced in the presence of GM-CSF and IL-4, and a maturation stimulus is added to the monocyte culture to obtain mature Dendritic Cells (DCs) suitable for therapy. TNF-α is the most common cytokine used for activating DCs and generating mature MDDC either alone or in combination with other cytokines. To compare effects of traditional cytokine cocktail (TNF-α + IL-1β) versus TLR4-agonist monophosphoryl lipid A on the viability, phenotype, cytokine profile and functionality of MDDC. The study included 32 individuals; twenty Acute Myeloid Leukaemia (AML) cases in complete remission and 12 healthy volunteers. They were divided into 3 groups: Group 1: control group: 12 subjects to measure the baseline levels of all markers in the monocytic preparation. Group 2: cytokine cocktail (TNF-α) group, which included 10 AML subjects. Group 3: MPLA group which included 10 AML subjects. TNF-α group showed higher expression of CD83 than MPLA group indicating higher capacity to induce DC maturation but both were similar in CD86, CCR7 and IL-10 expression. Preparation of dendritic cells from AML cases in remission and loading them with tumor peptides was successful. MPLA effect in DC maturation is comparable with traditional DC maturation cocktail.