Frequency of genes encoding erythromycin ribosomal methylases among Staphylococcus aureus clinical isolates with different D-phenotypes in Tehran, Iran
Macrolide, lincosamide and streptogramin type B (MLSB) antibiotics are important in the treatment of infections and existence of isolates with ability to resist against MLSB antibiotics is worrisome. In this cross sectional study, 101 isolates were collected from patients of five selected hospitals...
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Published in | Iranian journal of microbiology Vol. 8; no. 3; pp. 161 - 167 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Iran
Tehran University of Medical Sciences
01.06.2016
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Subjects | |
Online Access | Get full text |
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Summary: | Macrolide, lincosamide and streptogramin type B (MLSB) antibiotics are important in the treatment of
infections and existence of isolates with ability to resist against MLSB antibiotics is worrisome.
In this cross sectional study, 101
isolates were collected from patients of five selected hospitals in Tehran over a period of five months. Disk diffusion tests and differentiation between constitutive and inducible resistances were carried out by D-test. The presence of
and
genes were detected using PCR or multiplex PCR.
Out of 101
isolates, 58 (57.4%) were methicillin resistant and 57 (56.4%) expressed resistance to erythromycin. The prevalence of constitutive MLSB (cMLSB), inducible MLSB (iMLSB) and MS (Negative) phenotype in all erythromycin resistant isolates were 71.9, 26.3 and 1.7%, respectively. Out of all the erythromycin resistant isolates, 57.8% harbored both
and
genes which possessed constitutive resistance. 8.7% of the isolates contained
gene alone which possessed inducible resistance with D phenotype and 5.2% of isolates just contained
gene which had inducible resistance with D
phenotype.
gene was detected in 3.5% of the erythromycin resistant
isolates with constitutive resistance. None of the genes were detected among MS phenotypes.
In this study, most of
isolates carried both
and
genes and there was a significant relationship (
value ≤ 0.05) between different resistance phenotypes and
genes. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2008-3289 2008-4447 |