Effects of Gamma Irradiation on Changes in Chemical Composition and Antioxidant Activity of Euphorbia maculata Callus

• Published in: Plants (Basel) Vol. 13; no. 16; p. 2306
• Main Authors: Jeong, Gyeong Han, Kaur, Shubhpreet, Yoo, Youngchul, Ryu, Young Bae, Lee, Seo Jun, Jung, Kwang-Woo, Chung, Moon-Soo, Bai, Hyoung-Woo, Kim, Jin-Hong, Lee, Sungbeom, Kim, Tae Hoon, Chung, Byung Yeoup, Lee, Seung Sik
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 19.08.2024; MDPI
• Subjects: Antioxidants; Callus; Chemical activity; Chemical composition; Chromatography; Composition effects; Euphorbia; Euphorbia maculata; Flavonoids; Flowers & plants; Food irradiation; Functional foods & nutraceuticals; Gamma irradiation; High performance liquid chromatography; Investigations; Irradiation; Kinases; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Metabolism; Metabolites; Natural products; Phytochemicals; plant-derived callus; Quadrupoles; Radiation; radical scavenging; Scavenging; Statistical analysis; Sulfonic acid; UPLC-QTOF/MS; γ Radiation; UPLC-QTOF/MS; Euphorbia maculata; radical scavenging; plant-derived callus; gamma irradiation
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants13162306

In this study, we investigated the effects of gamma irradiation on the antioxidant activity and metabolite profiles of calli (PC3012). Gamma irradiation at various doses (0, 0.05, 0.5, and 10 kGy) significantly enhanced the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino- (3-ethylbenzothiazoline-6-sulphonic acid (ABTS ) radical scavenging activities of the callus extracts of PC3012 in a dose-dependent manner. High-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UPLC-Q-TOF/MS) analyses revealed that irradiation increased the lysophospholipid content, although no new antioxidant compounds were formed. Furthermore, a PLS-DA analysis revealed evident metabolic differences between non-irradiated and irradiated samples, which were further verified by statistical validation. These findings suggest that gamma irradiation induces specific biochemical modifications that enhance the bioactive properties of PC3012 calli. This technology exhibits potential for utilization in the natural product and food sectors, particularly in the development of functional foods and nutraceuticals with improved health benefits.