An Evaluation of the Cytocompatibility of Endodontic Bioceramics in Human Periodontal-Ligament-Derived Cells

• Published in: Journal of functional biomaterials Vol. 15; no. 8; p. 231
• Main Authors: Aka, Asuka, Matsuura, Takashi, Yoshimura, Atsutoshi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 19.08.2024; MDPI
• Subjects: Absorbance; Bioceramics; Biocompatibility; Consistency; cytocompatibility; endodontic bioceramics; Endodontics; human periodontal-ligament-derived cells; Hypotheses; Ligaments; MTA Flow White; MTA Repair HP; Nishika Canal Sealer BG multi; Pilot projects; Root canals; Sealers; Sealing compounds; Teeth; United States > US; Japan; cytocompatibility; Nishika Canal Sealer BG multi; endodontic bioceramics; MTA Flow White; MTA Repair HP; human periodontal-ligament-derived cells
• ISSN: 2079-4983; 2079-4983
• DOI: 10.3390/jfb15080231

The present study evaluated the cytocompatibility of three endodontic bioceramics in human periodontal-ligament-derived cells (hPDLCs): MTA Repair HP (HP), MTA Flow White (F), and Nishika Canal Sealer BG multi (BG). In addition, we also evaluated the effect of the powder-liquid (paste) ratio of F and BG on cytocompatibility. Discs of endodontic bioceramics (diameter = 8 mm, thickness = 1 mm) were prepared with HP, F, and BG. hPDLCs obtained from extracted teeth and cultured for three to five passages were used in the experiment. The prepared discs were placed at the bottom of a 48-well plate, seeded with hPDLCs at 100,000 cells/well, cultured for 7 or 28 days, and subjected to a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay. hPDLCs cultured without any discs were used as a negative control (NC) group. Discs made of F or BG mixed in three different consistencies were also used in this experiment. The absorbance values at days 7 and 28 were high in the order of HP > NC > BG > F. Furthermore, F or BG with higher consistency showed higher absorbance values. MTA Repair HP had the highest cytocompatibility among the three materials. Furthermore, it also showed that higher consistency improved cytocompatibility.