Supragingival microbiome variations and the influence of Candida albicans in adolescent orthodontic patients with gingivitis

• Published in: Journal of oral microbiology Vol. 16; no. 1; p. 2366056
• Main Authors: Yang, Hao, Ma, Yansong, Gao, Hongyu, Xie, Xianju, Wang, Hongmei, Li, Xiaowei, Bai, Yuxing
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 2024; Taylor & Francis Group
• Subjects: candida albicans; Fixed orthodontic treatment; gingivitis; microbiome diversity; supragingival plaque; microbiome diversity; supragingival plaque; gingivitis; candida albicans; Fixed orthodontic treatment
• ISSN: 2000-2297; 2000-2297
• DOI: 10.1080/20002297.2024.2366056

Gingivitis is a prevalent complication in adolescents undergoing fixed orthodontic treatments. However, changes in the supragingival microbiome associated with gingivitis and the impact of remain elusive. Therefore, we investigated supragingival microbiome discrepancy and colonization in adolescent orthodontic patients with gingivitis. Dental plaques were collected from 30 gingivitis patients and 24 healthy adolescents, all undergoing fixed orthodontic treatment. The supragingival microbiome composition was analyzed using 16S rRNA sequencing. colonization was determined using fungal culture and real-time quantitative polymerase chain reaction. Our analysis revealed significantly heightened microbial diversity in the Gingivitis group. Notably, patients with gingivitis exhibited an enrichment of periodontal pathogens, such as , , , and . Additionally, 33% of the gingivitis patients tested positive for , exhibiting significantly elevated levels of absolute abundance, while all healthy patients tested negative. Significant differences in microbial composition were also noted between -positive and -negative samples in the Gingivitis group. Significant disparities were observed in the supragingival microbiome of adolescent orthodontic patients with and without gingivitis. The presence of in the supragingival plaque may alter the microbiome composition and potentially contribute to gingivitis pathogenesis.