ALCAM-mediated cDC1 CD8 T cells interactions are suppressed in advanced lung tumors

• Published in: Oncoimmunology Vol. 13; no. 1; p. 2367843
• Main Authors: Morosi, Luciano G, Piperno, Giulia M, López, Lucía, Amadio, Roberto, Joshi, Sonal, Rustighi, Alessandra, Del Sal, Giannino, Benvenuti, Federica
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 2024; Taylor & Francis Group
• Subjects: Activated-Leukocyte Cell Adhesion Molecule; ALCAM; Animals; Antigens, CD - genetics; Antigens, CD - immunology; Antigens, CD - metabolism; Brief Report; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - immunology; Carcinoma, Non-Small-Cell Lung - metabolism; Carcinoma, Non-Small-Cell Lung - pathology; CD8-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - metabolism; cDC1; Cell Adhesion Molecules, Neuronal - genetics; Cell Adhesion Molecules, Neuronal - metabolism; Cell Communication - immunology; Dendritic Cells - immunology; Dendritic Cells - metabolism; Fetal Proteins - genetics; Fetal Proteins - metabolism; Humans; immunological synapse; lung cancer; Lung Neoplasms - genetics; Lung Neoplasms - immunology; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; Mice; NSCLC; immunological synapse; lung cancer; cDC1; ALCAM; NSCLC
• ISSN: 2162-402X; 2162-4011; 2162-402X
• DOI: 10.1080/2162402X.2024.2367843

Conventional type 1 dendritic cells (cDC1) are critical regulators of anti-tumoral T-cell responses. The structure and abundance of intercellular contacts between cDC1 and CD8 T cells in cancer tissues is important to determine the outcome of the T-cell response. However, the molecular determinants controlling the stability of cDC1-CD8 interactions during cancer progression remain poorly investigated. Here, we generated a genetic model of non-small cell lung cancer crossed to a fluorescent cDC1 reporter (KP-XCR1 ) to allow the detection of cDC1-CD8T cell clusters in tumor tissues across tumor stages. We found that cDC1-CD8 clusters are abundant and productive at the early stages of tumor development but progressively diminish in advanced tumors. Transcriptional profiling and flow cytometry identified the adhesion molecule ALCAM/CD166 (Activated Leukocyte Cell Adhesion Molecule, ligand of CD6) as highly expressed by lung cDC1 and significantly downregulated in advanced tumors. Analysis of human datasets indicated that is downregulated in non-small cell lung cancer and its expression correlates to better prognosis. Mechanistically, triggering ALCAM on lung cDC1 induces cytoskeletal remodeling and contact formation whereas its blockade prevents T-cell activation. Together, our results indicate that ALCAM is important to stabilize cDC1-CD8 interactions at early tumor stages, while its loss in advanced tumors contributes to immune evasion.