20S-Protopanaxadiol, an aglycosylated ginsenoside metabolite, induces hepatic stellate cell apoptosis through liver kinase B1–AMP-activated protein kinase activation

• Published in: Journal of ginseng research Vol. 41; no. 3; pp. 392 - 402
• Main Authors: Park, Sang Mi, Jung, Eun Hye, Kim, Jae Kwang, Jegal, Kyung Hwan, Park, Chung A, Cho, Il Je, Kim, Sang Chan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.07.2017; 고려인삼학회; Elsevier
• Subjects: 20S-protopanaxadiol (PPD); AMP-activated protein kinase (AMPK); apoptosis; hepatic stellate cells (HSCs); liver kinase B1 (LKB1); 20S-protopanaxadiol (PPD); apoptosis; liver kinase B1 (LKB1); hepatic stellate cells (HSCs); AMP-activated protein kinase (AMPK)
• ISSN: 1226-8453; 2093-4947
• DOI: 10.1016/j.jgr.2017.01.012

Previously, we reported that Korean Red Ginseng inhibited liver fibrosis in mice and reduced the expressions of fibrogenic genes in hepatic stellate cells (HSCs). The present study was undertaken to identify the major ginsenoside responsible for reducing the numbers of HSCs and the underlying mechanism involved. Using LX-2 cells (a human immortalized HSC line) and primary activated HSCs, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assays were conducted to examine the cytotoxic effects of ginsenosides. H2O2 productions, glutathione contents, lactate dehydrogenase activities, mitochondrial membrane permeabilities, apoptotic cell subpopulations, caspase-3/-7 activities, transferase dUTP nick end labeling (TUNEL) staining, and immunoblot analysis were performed to elucidate the molecular mechanism responsible for ginsenoside-mediated cytotoxicity. Involvement of the AMP-activated protein kinase (AMPK)-related signaling pathway was examined using a chemical inhibitor and small interfering RNA (siRNA) transfection. Of the 11 ginsenosides tested, 20S-protopanaxadiol (PPD) showed the most potent cytotoxic activity in both LX-2 cells and primary activated HSCs. Oxidative stress-mediated apoptosis induced by 20S-PPD was blocked by N-acetyl-l-cysteine pretreatment. In addition, 20S-PPD concentration-dependently increased the phosphorylation of AMPK, and compound C prevented 20S-PPD-induced cytotoxicity and mitochondrial dysfunction. Moreover, 20S-PPD increased the phosphorylation of liver kinase B1 (LKB1), an upstream kinase of AMPK. Likewise, transfection of LX-2 cells with LKB1 siRNA reduced the cytotoxic effect of 20S-PPD. Thus, 20S-PPD appears to induce HSC apoptosis by activating LKB1–AMPK and to be a therapeutic candidate for the prevention or treatment of liver fibrosis.