Widely targeted metabolomics reveals the phytoconstituent changes in Platostoma palustre leaves and stems at different growth stages

• Published in: Frontiers in plant science Vol. 15; p. 1378881
• Main Authors: Huang, Suhua, Chen, Zhining, Chen, Hao, Quan, Changqian, Xu, Meihua, Wei, Fan, Tang, Danfeng
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 18.06.2024
• Subjects: metabolic pathways; phytoconstituents changes; Plant Science; Platostoma palustre; potential markers; widely targeted metabolomics; Platostoma palustre; phytoconstituents changes; potential markers; widely targeted metabolomics; metabolic pathways
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2024.1378881

is an important edible and medicinal plant. To gain a comprehensive and clear understanding of the variation patterns of metabolites in , we employed the UPLC-MS platform along with widely targeted metabolomics techniques to analyze the metabolites in the stems and leaves of at different stages. Our results revealed a total of 1228 detected metabolites, including 241 phenolic acids, 203 flavonoids, 152 lipids, 128 terpenes, 106 amino acids, 79 organic acids, 74 saccharides, 66 alkaloids, 44 lignans, etc. As the growth time increased, the differential metabolites (DAMs) mainly enriched in leaves were terpenoids, phenolic acids, and lipids, while the DAMs primarily enriched in stems were terpenoids. Compared to stems, there were more differential flavonoids in leaves, and saccharides and flavonoids were significantly enriched in leaves during the S1 and S2 stages. Additionally, we identified 13, 10, and 23 potential markers in leaf, stem, and leaf vs. stem comparison groups. KEGG enrichment analysis revealed that arginine biosynthesis was the common differential metabolic pathway in different growth stages and tissues. Overall, this study comprehensively analyzed the metabolic profile information of , serving as a solid foundation for its further development and utilization.