Evaluation of bioactive extract nanoparticles on pulp stem cell behavior relevant to dental care using chemical composition of gelatin-Arabian gum nano polymer

• Published in: Iranian journal of basic medical sciences Vol. 27; no. 8; pp. 975 - 984
• Main Authors: Tan, Xiaoni, Zhang, Moli, Tu, BiBo
• Format: Journal Article
• Language: English
• Published: Iran Mashhad University of Medical Sciences 01.01.2024
• Subjects: bioactive plant extracts; chemical stability; Dental care; gelatin polymer scaffold; Metabolism; migration; Original; Polymers; proliferation; pulp stem cells; Gelatin polymer scaffold; Proliferation; Chemical stability; Migration; Pulp stem cells; Bioactive plant extracts
• ISSN: 2008-3866; 2008-3874
• DOI: 10.22038/IJBMS.2024.76467.16548

This study aimed to investigate the impact of bioactive plant extracts on the proliferation and migration of dental pulp stem cells (DPSCs) and their potential implications for dental care, focusing on the nurse-caring aspect. TDPSCs were cultured on gelatin polymer scaffolds mimicking the extracellular matrix (ECM) environment. Bioactive plant extracts with antibacterial, anti-inflammatory, and anti-oxidant properties were incorporated into the gelatin polymer at concentrations ranging from 0.1% to 2.0%. Proliferation and migration assays were performed, considering nurse-caring practices during the experiments. Treatment with specific bioactive plant extracts significantly enhanced DPSC proliferation, showing a 2.5-fold increase compared to the control groups. The migration assay revealed a substantial increase in cell migration distance, with treated cells covering an average distance of 400-500 μm compared to 220-260 μm in the control group. Treated cells also exhibited improved viability and metabolic activity, with a 30% increase in cell viability and a 10-20% increase in metabolic activity compared to the control group. This study demonstrates that bioactive plant extracts have the potential to enhance DPSC proliferation, migration, viability, and metabolic activity. These findings support the use of these extracts in dental care, benefiting from the nurse-caring practices.