Molecular diagnostics and variability of longidorid nematodes
PCR-RFLP and sequencing approaches of ribosomal DNA are being used to study taxonomy, molecular identification and phylogeny of plant parasitic nematodes. In this paper, we discuss on the usefulness of ITS PCR-RFLP analysis to differentiate among longidorid species. In addition, we examined how well...
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Published in | Phytopathologia mediterranea Vol. 43; no. 2; pp. 263 - 267 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Firenze University Press
01.08.2004
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Subjects | |
Online Access | Get full text |
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Summary: | PCR-RFLP and sequencing approaches of ribosomal DNA are being used to study taxonomy, molecular identification and phylogeny of plant parasitic nematodes. In this paper, we discuss on the usefulness of ITS PCR-RFLP analysis to differentiate among longidorid species. In addition, we examined how well ITS PCR-RFLP differentiated between longidorid species, and how well sequencing of two different ribosomal regions, the ITS containing region and D1-D2 domains of the 26S rDNA, was able to infer phylogenetic relationships among those same species. These methods and their advantages in identifying longidorids and establishing their phylogenetic relationships are examined and discussed
[La PCR-RFLP e il sequenziamento dell´RNA ribosomiale sono utilizzati per studiare la tassonomia, l´identificazione molecolare e la filogenesi dei nematodi parassiti delle piante. In questo lavoro discutiamo l´utilità dell´analisi PCR-RFLP degli ITS per distinguere le specie di longodoridi. Inoltre, abbiamo esaminato come la PCR-RFLP degli ITS differenziava bene le specie di longidoridi e come il sequenziamento di due regioni ribosomiali diverse, la regione contenente gli ITS e i domini D1-D2 dell´rRNA 26S, era in grado di presupporre rapporti filogenetici fra quelle stesse specie. Sono esaminati e discussi questi metodi e i loro vantaggi nell´identificazione dei longidoridi e nello stabilire i loro rapporti filogenetici.] |
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Bibliography: | 2005601324 L10 H10 L60 |
ISSN: | 0031-9465 1593-2095 |
DOI: | 10.14601/Phytopathol_Mediterr-1751 |