Oxidative Stress Assessment in Different Ethnic Groups of Girls with Exogenous Constitutional Obesity Complicated by Non-Alcoholic Fatty Liver Disease

• Published in: International journal of biomedicine Vol. 9; no. 3; pp. 223 - 227
• Main Authors: Marina A. Darenskaya, Lyubov V. Rychkova, Larisa A. Zhdanova, Lyudmila A. Grebenkina, Oksana A. Gavrilova, Elena V. Osipova, Natalya V. Semenova, Sergey I. Kolesnikov, Lyubov I. Kolesnikova
• Format: Journal Article
• Language: English
• Published: International Medical Research and Development Corporation 01.09.2019
• Subjects: adolescent; non-alcoholic fatty liver disease; obesity; oxidative stress
• ISSN: 2158-0510; 2158-0529
• DOI: 10.21103/Article9(3)_OA7

The purpose of this study was to assess the lipid peroxidation (LPO) and antioxidative defense (AOD) indicators in different ethnic groups of adolescent girls with exogenous constitutional obesity (ECO) and non-alcoholic fatty liver disease (NAFLD). Materials and Methods: A total of 20 Mongoloid teenage girls and 20 Caucasian teenage girls with NAFLD (Clinical group 1 and Clinical group 2, respectively) on the background of ECO of the first degree were examined. For comparison, data of 28 healthy Mongoloid teenage girls (Control group 1) and 20 Caucasian teenage girls (Control group 2) were used. The plasma level of antioxidant parameters (total antioxidant activity [TTA], SOD activity, α-tocopherol and retinol) and primary/secondary products of LPO (conjugated dienes [CD], ketodienes and conjugated trienes [KD-CT], and thiobarbituric acid reactive substances [TBARS]) were determined using spectrophotometric and fluorometric methods. Results: Evaluation of the activity of LPO reactions in Clinical group 1 indicated an increase in the content of CD, KD-CT, and TBARS relative to Control group 1. In Clinical group 2, we found an increased CD content (P=0.0463) relative to Control group 2. Interethnic differences resulted in elevated levels of KD-CT in Clinical group 1 compared to Clinical group 2.In the parameters of the AOD system, we observed reduced levels of TAA, retinol and glutathione-S-transferase (G-S-T) activity in Clinical group 2 compared to Control group 2. In Clinical group 1, we observed reduced levels of α-tocopherol, retinol and G-S-T activity compared to Control group 1. There were no differences in the parameters of the AOD system between Clinical group 1 and Clinical group 2. Conclusion: The results obtained indicate high activity of LPO processes and severe deficiency of antioxidant factors in Mongoloid girls with ECO and NAFLD in comparison with Caucasian girls with obesity and NAFLD and the control group. The obtained results allow us to recommend administration of antioxidant drugs in addition to basic therapy in comprehensive treatment of patients with NAFLD.