Polyphenol Contents, Antibacterial and Antioxidant Effects of Four Palestinian Honey Samples, and their Anticancer Effects on Human Breast Cancer Cells

The phenolic compounds of four Palestinian honey samples (PH1-PH4) and their antibacterial effects as well as their cytotoxic, cytostatic, and antimigration effects in human breast cancer cell line (MDA) were evaluated here. HPLC analysis of PH2 (Cornflower), PH3 (Milk thistle), and PH4 (Ziziphus) r...

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Published inJournal of pure & applied microbiology : an international research journal of microbiology Vol. 18; no. 2; pp. 1372 - 1385
Main Authors Abu-Farich, Basheer, Hamarshi, Hadeel, Masalha, Mahmud, Aboulghazi, Abderrazak, Kmail, Abdalsalam, Ouassete, Mohammed El, Imtara, Hamada, Lyoussi, Badiaa, Saad, Bashar
Format Journal Article
LanguageEnglish
Published Oriental Scientific Publishing Company 01.06.2024
Journal of Pure and Applied Microbiology
Subjects
Analysis
antibacterial
anticancer
antimigration
antioxidant
Antioxidants
Breast cancer
Cancer
Care and treatment
cytostatic
cytotoxic
Ellagic acid
Escherichia coli
Methicillin
Staphylococcus aureus
Tetracycline
Tetracyclines
Palestinian Territories
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Summary:The phenolic compounds of four Palestinian honey samples (PH1-PH4) and their antibacterial effects as well as their cytotoxic, cytostatic, and antimigration effects in human breast cancer cell line (MDA) were evaluated here. HPLC analysis of PH2 (Cornflower), PH3 (Milk thistle), and PH4 (Ziziphus) revealed 15 phenolic compounds, namely, caffeic acid, carvacrol, chrysin, ellagic acid, galangin, gallic acid, kaempferol, p-coumaric acid, pinobanksin, pinocembrin, protocatechuic acid, quercetin, rutin, salicylic acid, and silydamin. The minimum inhibitory concentration (MIC) method applied to Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus), and Escherichia coli (E. coli). A strong positive correlation was detected between antimicrobial activity (E.. coli) and p-coumaric acid, quercetin, and silydamin. IC50 values for DPPH neutralization varied from 0.19 w/w% to 10 w/w%. The potential anticancer properties of the honey samples were evaluated on MDA cells. Samples PH2 and PH3 demonstrated cytostatic activity, reducing cell viability by about 43% at non-toxic concentration of 4 mg/mL. The cytostatic effects were strongly correlated with the presence of caffeic acid, chrysin, protocatechuic acid, rutin, and salicylic acid (p < 0.01). Moreover, the cell migration rate was significantly reduced (by up to 85%) with PH2 and PH3 compared to untreated cells (p < 0.05). A strong positive correlation was observed between the cytostatic effects of the concentration of carvacrol and Pinocembrin (p < 0.01). Our findings validate honey's antibacterial properties and suggest its anticancer benefits may stem from cytostatic and antimigration effects. Keywords: Antibacterial, Antioxidant, Anticancer, Cytostatic, Cytotoxic, Antimigration
ISSN:0973-7510
2581-690X
DOI:10.22207/JPAM.18.2.60