SCREENING FREEZE-DRYING CRYOPROTECTANTS FOR SACCHAROMYCES BOULARDII BY PLACKETT-BURMAN DESIGN

As a lyophilized product, Saccharomyces boulardii has been commonly used to treat diarrhea in adults. However, there are few studies focusing on the preparation of its freeze-drying powder. This paper investigated the effect of lyoprotectants on the freeze-drying survival rate and the number of viab...

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Bibliographic Details
Published inThe Annals of the University Dunarea de Jos of Galati. Fascicle VI, Food technology Vol. 40; no. 2; pp. 83 - 97
Main Authors Chen, He, Lei, Zhangteng, Hong, Haiping, Zhai, Yaling, Huang, Dan
Format Journal Article
LanguageEnglish
Published Galati Universityi Dunarea de Jos of Galati 01.01.2016
Galati University Press
Subjects
Antibiotics
Carbohydrates
Design of experiments
Diarrhea
freeze-drying
Lactose
lyoprotectants
Microorganisms
Permeability
PlackettBurman design
Prebiotics
Probiotics
Proteins
Reagents
Saccharomyces boulardii
Science
Sodium
Statistical analysis
Studies
Variables
Yeast
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Summary:As a lyophilized product, Saccharomyces boulardii has been commonly used to treat diarrhea in adults. However, there are few studies focusing on the preparation of its freeze-drying powder. This paper investigated the effect of lyoprotectants on the freeze-drying survival rate and the number of viable cells for vacuum freezedried powder of S. boulardii. Single factor experiment and Plackett-Burman design were conducted to obtain the optimal compound lyoprotectant formulations. The result showed that lactose, trehalose and sodium glutamate could significantly enhance the freeze-drying survival rate of S. boulardii. Meanwhile, all these three lyoprotectants showed positive effect on the freeze-drying survival rate of the yeast, and the optimal lyoprotectant composition for S. boulardii was as follows: 18g/100mL lactose, 18g/100mL trehalose and 3g/100mL sodium glutamate.
ISSN:1843-5157
2068-259X