Investigating the Effect of Bacilli and Lactic Acid Bacteria on Water Quality, Growth, Survival, Immune Response, and Intestinal Microbiota of Cultured Litopenaeus vannamei

• Published in: Animals (Basel) Vol. 14; no. 18; p. 2676
• Main Authors: Vega-Carranza, Ana Sofía, Escamilla-Montes, Ruth, Fierro-Coronado, Jesús Arturo, Diarte-Plata, Genaro, Guo, Xianwu, García-Gutiérrez, Cipriano, Luna-González, Antonio
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 14.09.2024; MDPI
• Subjects: Anticoagulants; Aquaculture; Bacteria; Bioassays; Enzymes; Feeds; Immune system; intestinal microbiota; Litopenaeus vannamei; Microbiota; Nitrates; nitrifying bacteria; Oxidation; Probiotics; Salinity; Seawater; Water quality; United States > US; Mexico; intestinal microbiota; Litopenaeus vannamei; water quality; nitrifying bacteria
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani14182676

Shrimp is one of the most important aquaculture industries. Therefore, we determined the effect of nitrifying-probiotic bacteria on water quality, growth, survival, immune response, and intestinal microbiota of cultured without water exchange. In vitro, only used total ammonia nitrogen (TAN), nitrites, and nitrates since nitrogen bubbles were produced. TAN decreased significantly in the treatments with and and , but no differences were observed in nitrites. Nitrates were significantly higher in the treatments with bacteria. The final weight was higher only with bacilli and bacilli and LAB treatments. The survival of shrimp in the bacterial treatments increased significantly, and superoxide anion increased significantly only in lactic acid bacteria (LAB) treatment. The activity of phenoloxidase decreased significantly in the treatments with bacteria compared to the control. Shrimp treated with bacilli in the water showed lower species richness. The gut bacterial community after treatments was significantly different from that of the control. Linoleic acid metabolism was positively correlated with final weight and superoxide anion, whereas quorum sensing was correlated with survival. Thus, bacilli and LAB in the water of hyperintensive culture systems act as heterotrophic nitrifers, modulate the intestinal microbiota and immune response, and improve the growth and survival of shrimp. This is the first report on and identified as nitrifying bacteria.